BMRB Entry 52084
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52084
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
All files associated with the entry
Citation: Yang, Min June; Park, Chin-Ju. "Backbone assignment of the intrinsically disordered N-terminal region of Bloom syndrome protein" J. Korean Mag. Res. Soc. 27, 17-22 (2023).
Assembly members:
entity_1, polymer, 84 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET His 6 GST LIC
Entity Sequences (FASTA):
entity_1: SNALTEEQKDDSEWLSSDVI
CIDDGPIAEVHINEDAQESD
SLKTHLEDERDNSEKKKNLE
EAELHSTEKVPCIEFDDDDY
DTDF
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 242 |
| 15N chemical shifts | 72 |
| 1H chemical shifts | 72 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | BLM 220-300 | 1 |
Entities:
Entity 1, BLM 220-300 84 residues - Formula weight is not available
| 1 | SER | ASN | ALA | LEU | THR | GLU | GLU | GLN | LYS | ASP | ||||
| 2 | ASP | SER | GLU | TRP | LEU | SER | SER | ASP | VAL | ILE | ||||
| 3 | CYS | ILE | ASP | ASP | GLY | PRO | ILE | ALA | GLU | VAL | ||||
| 4 | HIS | ILE | ASN | GLU | ASP | ALA | GLN | GLU | SER | ASP | ||||
| 5 | SER | LEU | LYS | THR | HIS | LEU | GLU | ASP | GLU | ARG | ||||
| 6 | ASP | ASN | SER | GLU | LYS | LYS | LYS | ASN | LEU | GLU | ||||
| 7 | GLU | ALA | GLU | LEU | HIS | SER | THR | GLU | LYS | VAL | ||||
| 8 | PRO | CYS | ILE | GLU | PHE | ASP | ASP | ASP | ASP | TYR | ||||
| 9 | ASP | THR | ASP | PHE |
Samples:
sample_1: BLM 220-300, [U-100% 13C; U-100% 15N], 500 uM
sample_conditions_1: ionic strength: 50 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
Software:
PINE - chemical shift assignment
NMR spectrometers:
- Bruker AVANCE III 800 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks