BMRB Entry 52068

Name: Chemical shift assignment of the ClpL-N-terminal domain from Listeria monocytogenes
Published: 2024-04-05

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52068

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

Chemical shift assignment of the ClpL-N-terminal domain from Listeria monocytogenes

Deposition date:

2023-08-04

Original release date:

2024-04-05

Authors:

Hennig, Janosch; Hollmann, Nele

Citation:

Citation: Bohl, Valentin; Hollmann, Nele; Melzer, Tobias; Katikaridis, Panagiotis; Meins, Lena; Simon, Bernd; Fleming, Dirk; Sinning, Irmgard; Hennig, Janosch; Mogk, Axel. "The Listeria monocytogenes persistence factor ClpL is a potent stand-alone disaggregase" eLife 12, 92746-92746 (2024).
PubMed: 38598269

Assembly members:

Assembly members:
entity_1, polymer, 69 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Listeria monocytogenes Taxonomy ID: 1639 Superkingdom: Bacteria Kingdom: not available Genus/species: Listeria monocytogenes

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET24a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MANFYGNDPFFNNDMDDVFN QLFRRMDNQNSERARYLVNG QSLTPDEFTQYRATGKLPQN NLEHHHHHH

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	214
15N chemical shifts	55
1H chemical shifts	355

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	ClpL-NTD	1

Entities:

Entity 1, ClpL-NTD 69 residues - Formula weight is not available

1

MET

ALA

ASN

PHE

TYR

GLY

ASN

ASP

PRO

PHE

2

PHE

ASN

ASP

MET

ASP

VAL

PHE

ASN

3

GLN

LEU

PHE

ARG

MET

ASP

ASN

GLN

ASN

4

SER

GLU

ARG

ALA

ARG

TYR

LEU

VAL

ASN

GLY

5

GLN

SER

LEU

THR

PRO

ASP

GLU

PHE

THR

GLN

6

TYR

ARG

ALA

THR

GLY

LYS

LEU

PRO

GLN

ASN

7

ASN

LEU

GLU

HIS

Samples:

sample_1: ClpL-NTD, [U-99% 13C; U-99% 15N], 410 uM; sodium phosphate 50 mM; potassium chloride 50 mM; dithiothreitol 2 mM

sample_conditions_1: ionic strength: 50 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
2D 1H-13C HMQC	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-13C HMQC	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1

Software:

CARA - chemical shift assignment

NMR spectrometers:

Bruker AVANCE III 600 MHz
Bruker AVANCE III 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks