BMRB Entry 52064

Name: NMR assignment of the N-terminal region of the Translocated Intimin Receptor (res. 61 to 200)
Published: 2024-02-14

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52064

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

NMR assignment of the N-terminal region of the Translocated Intimin Receptor (res. 61 to 200)

Deposition date:

2023-07-31

Original release date:

2024-02-14

Authors:

Hernandez, Guillem; Vieira, Marta; Zhong, Qiyun; Arbesu, Miguel; Veloso, Tiago; Gomes, Tiago; Monteiro, Hugo; Frankel, Gad; Zanzoni, Andreas; Cordeiro, Tiago

Citation:

Citation: Vieira, Marta; Hernandez, Guillem; Zhong, Qiyun; Arbesu, Miguel; Veloso, Tiago; Gomes, Tiago; Martins, Maria; Monteiro, Hugo; Frazao, Carlos; Frankel, Gad; Zanzoni, Andreas; Cordeiro, Tiago. "The pathogen-encoded signalling receptor Tir exploits host-like intrinsic disorder for infection" Commun. Biol. 7, 179-179 (2024).
PubMed: 38351154

Assembly members:

Assembly members:
entity_1, polymer, 149 residues, 16000 Da.

Natural source:

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pHTP8

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GVDSRDIPGLPTNPSRLAAA TSETCLLGGFEVLHDKGPLD ILNTQIGPSAFRVEVQADGT HAAIGEKNGLEVSVTLSPQE WSSLQSIDTEGKNRFVFTGG RGGSGHPMVTVASDIAEART KILAKLDPDNHGGRQPKDVD TWSHPQFEK

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	446
15N chemical shifts	116
1H chemical shifts	584

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	NS-Tir, chain A	1
2	NS-Tir, chain B	1

Entities:

Entity 1, NS-Tir, chain A 149 residues - 16000 Da.

1

GLY

VAL

ASP

SER

ARG

ASP

ILE

PRO

GLY

LEU

2

PRO

THR

ASN

PRO

SER

ARG

LEU

ALA

3

THR

SER

GLU

THR

CYS

LEU

GLY

PHE

4

GLU

VAL

LEU

HIS

ASP

LYS

GLY

PRO

LEU

ASP

5

ILE

LEU

ASN

THR

GLN

ILE

GLY

PRO

SER

ALA

6

PHE

ARG

VAL

GLU

VAL

GLN

ALA

ASP

GLY

THR

7

HIS

ALA

ILE

GLY

GLU

LYS

ASN

GLY

LEU

8

GLU

VAL

SER

VAL

THR

LEU

SER

PRO

GLN

GLU

9

TRP

SER

LEU

GLN

SER

ILE

ASP

THR

GLU

10

GLY

LYS

ASN

ARG

PHE

VAL

PHE

THR

GLY

11

ARG

GLY

SER

GLY

HIS

PRO

MET

VAL

THR

12

VAL

ALA

SER

ASP

ILE

ALA

GLU

ALA

ARG

THR

13

LYS

ILE

LEU

ALA

LYS

LEU

ASP

PRO

ASP

ASN

14

HIS

GLY

ARG

GLN

PRO

LYS

ASP

VAL

ASP

15

THR

TRP

SER

HIS

PRO

GLN

PHE

GLU

LYS

Samples:

sample_1: NS-Tir, [U-100% 13C; U-100% 15N], 1.3 mM; HEPES 50 mM; sodium chloride 150 mM; EDTA 1 mM; D2O 8%; DSS 20 uM

sample_conditions_1: ionic strength: 150 mM; pH: 6.5; pressure: 1 atm; temperature: 288 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CO)CACB	sample_1	isotropic	sample_conditions_1
2D 1H-15N TROSY	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN v4.6.2 - collection

NMR spectrometers:

Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks