BMRB Entry 52062

Title:
Backbone 1H and 15N Chemical Shift Assignments for the region 310-350 for TDP-43 C-terminal domain engineered variant 5M to A outside CR
Deposition date:
2023-07-30
Original release date:
2023-10-28
Authors:
Krishnashenoy Padmabai, Jayakrishna Shenoy; Mohanty, Priyesh; Rizuan, Azamat; Mercado Ortiz, Jose; Fawzi, Nicolas; Mittal, Jeetain
Citation:

Citation: Mohanty, Priyesh; Krishnashenoy Padmabai, Jayakrishna Shenoy; Rizuan, Azamat; Mercado Ortiz, Jose; Fawzi, Nicolas; Mittal, Jeetain. "A synergy between site-specific and transient interactions drives the phase separation of a disordered, low-complexity domain"  Proc. Natl. Acad. Sci. U. S. A. 120, e2305625120-e2305625120 (2023).
PubMed: 37579155

Assembly members:

Assembly members:
entity_1, polymer, 148 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pJ411

Data sets:
Data typeCount
15N chemical shifts39
1H chemical shifts39

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
15M to A IDR1

Entities:

Entity 1, 5M to A IDR 148 residues - Formula weight is not available

1   ASNARGGLNLEUGLUARGSERGLYARGPHE
2   GLYGLYASNPROGLYGLYPHEGLYASNGLN
3   GLYGLYPHEGLYASNSERARGGLYGLYGLY
4   ALAGLYLEUGLYASNASNGLNGLYSERASN
5   ALAGLYGLYGLYALAASNPHEGLYALAPHE
6   SERILEASNPROALAMETMETALAALAALA
7   GLNALAALALEUGLNSERSERTRPGLYMET
8   METGLYMETLEUALASERGLNGLNASNGLN
9   SERGLYPROSERGLYASNASNGLNASNGLN
10   GLYASNALAGLNARGGLUPROASNGLNALA
11   PHEGLYSERGLYASNASNSERTYRSERGLY
12   SERASNSERGLYALAALAILEGLYTRPGLY
13   SERALASERASNALAGLYSERGLYSERGLY
14   PHEASNGLYGLYPHEGLYSERSERALAASP
15   SERLYSSERSERGLYTRPGLYALA

Samples:

sample_1: 5M to A IDR TDP-43 mutant, [U-100% 15N], 20 uM

sample_conditions_1: ionic strength: 0 M; pH: 6.1; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

TOPSPIN - collection

CcpNMR - chemical shift assignment, data analysis

NMR spectrometers:

  • Bruker Avance 850 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks