BMRB Entry 52059

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52059

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Title:
Chemical shifts of hHR23A 2-363
Deposition date:
2023-07-29
Original release date:
2023-10-05
Authors:
Chen, Xiang; Walters, Kylie
Citation:

Citation: Chen, Xiang; Walters, Kylie. "1H, 15N, 13C backbone and side chain resonance assignments for hHR23a full-length"  Biomol. NMR Assignments ., .-..

Assembly members:

Assembly members:
entity_1, polymer, 363 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX-6p-1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: SAVTITLKTLQQQTFKIRME PDETVKVLKEKIEAEKGRDA FPVAGQKLIYAGKILSDDVP IRDYRIDEKNFVVVMVTKTK AGQGTSAPPEASPTAAPESS TSFPPAPTSGMSHPPPAARE DKSPSEESAPTTSPESVSGS VPSSGSSGREEDAASTLVTG SEYETMLTEIMSMGYERERV VAALRASYNNPHRAVEYLLT GIPGSPEPEHGSVQESQVSE QPATEAAGENPLEFLRDQPQ FQNMRQVIQQNPALLPALLQ QLGQENPQLLQQISRHQEQF IQMLNEPPGELADISDVEGE VGAIGEEAPQMNYIQVTPQE KEAIERLKALGFPESLVIQA YFACEKNENLAANFLLSQNF DDE

Data sets:
Data typeCount
13C chemical shifts1052
15N chemical shifts317
1H chemical shifts1693

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1hHR23A1

Entities:

Entity 1, hHR23A 363 residues - Formula weight is not available

1   SERALAVALTHRILETHRLEULYSTHRLEU
2   GLNGLNGLNTHRPHELYSILEARGMETGLU
3   PROASPGLUTHRVALLYSVALLEULYSGLU
4   LYSILEGLUALAGLULYSGLYARGASPALA
5   PHEPROVALALAGLYGLNLYSLEUILETYR
6   ALAGLYLYSILELEUSERASPASPVALPRO
7   ILEARGASPTYRARGILEASPGLULYSASN
8   PHEVALVALVALMETVALTHRLYSTHRLYS
9   ALAGLYGLNGLYTHRSERALAPROPROGLU
10   ALASERPROTHRALAALAPROGLUSERSER
11   THRSERPHEPROPROALAPROTHRSERGLY
12   METSERHISPROPROPROALAALAARGGLU
13   ASPLYSSERPROSERGLUGLUSERALAPRO
14   THRTHRSERPROGLUSERVALSERGLYSER
15   VALPROSERSERGLYSERSERGLYARGGLU
16   GLUASPALAALASERTHRLEUVALTHRGLY
17   SERGLUTYRGLUTHRMETLEUTHRGLUILE
18   METSERMETGLYTYRGLUARGGLUARGVAL
19   VALALAALALEUARGALASERTYRASNASN
20   PROHISARGALAVALGLUTYRLEULEUTHR
21   GLYILEPROGLYSERPROGLUPROGLUHIS
22   GLYSERVALGLNGLUSERGLNVALSERGLU
23   GLNPROALATHRGLUALAALAGLYGLUASN
24   PROLEUGLUPHELEUARGASPGLNPROGLN
25   PHEGLNASNMETARGGLNVALILEGLNGLN
26   ASNPROALALEULEUPROALALEULEUGLN
27   GLNLEUGLYGLNGLUASNPROGLNLEULEU
28   GLNGLNILESERARGHISGLNGLUGLNPHE
29   ILEGLNMETLEUASNGLUPROPROGLYGLU
30   LEUALAASPILESERASPVALGLUGLYGLU
31   VALGLYALAILEGLYGLUGLUALAPROGLN
32   METASNTYRILEGLNVALTHRPROGLNGLU
33   LYSGLUALAILEGLUARGLEULYSALALEU
34   GLYPHEPROGLUSERLEUVALILEGLNALA
35   TYRPHEALACYSGLULYSASNGLUASNLEU
36   ALAALAASNPHELEULEUSERGLNASNPHE
37   ASPASPGLU

Samples:

sample_1: hHR23A 2-363, [U-100% 13C; U-100% 15N], 0.5 mM; sodium phosphate 20 mM; sodium chloride 100 mM; DTT 2 mM; sodium azide 0.1%

sample_2: hHR23A 2-363, [U-100% 13C; U-100% 15N; U-50% 2H], 0.5 mM; sodium phosphate 20 mM; sodium chloride 100 mM; DTT 2 mM; sodium azide 0.1%

sample_3: hHR23A 2-363, [U-100% 13C], 0.5 mM; sodium phosphate 20 mM; sodium chloride 100 mM; DTT 2 mM; sodium azide 0.1%

sample_conditions_1: ionic strength: 120 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_2isotropicsample_conditions_1
3D 15N-separated NOESYsample_2isotropicsample_conditions_1
2D 1H-13C HSQCsample_3isotropicsample_conditions_1
3D 13C-separated NOESYsample_3isotropicsample_conditions_1
2D 1H-1H NOESYsample_3isotropicsample_conditions_1

Software:

VNMR - collection

NMRPipe - processing

XEASY - chemical shift assignment, peak picking

TALOS+ - data analysis

NMR spectrometers:

  • Varian INOVA 800 MHz
  • Varian INOVA 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks