BMRB Entry 52046

Name: Assignment of PRPF40A N-terminal extended WW12 construct
Published: 2024-03-28

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52046

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Assignment of PRPF40A N-terminal extended WW12 construct

Deposition date:

2023-07-20

Original release date:

2024-03-28

Authors:

Martinez-Lumbreras, Santiago; Sattler, Michael

Citation:

Citation: Martinez-Lumbreras, Santiago; Trager, Lena; Mulorz, Miriam M.; Payr, Marco; Dikaya, Varvara; Hipp, Clara; Konig, Julian; Sattler, Michael. "Intramolecular autoinhibition regulates the selectivity of PRPF40A tandem WW domains for proline-rich motifs" Nat. Commun. 15, 3888-3888 (2024).
PubMed: 38719828

Assembly members:

Assembly members:
entity_1, polymer, 183 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pETM11

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GAMGHPGMHYAPMGMHPMGQ RANMPPVPHGMMPQMMPPMG GPPMGQMPGMMSSVMPGMMM SHMSQASMQPALPPGVNSMD VAAGTASGAKSMWTEHKSPD GRTYYYNTETKQSTWEKPDD LKTPAEQLLSKCPWKEYKSD SGKPYYYNSQTKESRWAKPK ELEDLEGYQNTIVAGSLITK SNL

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	183
15N chemical shifts	129
1H chemical shifts	252

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	NterWW12	1

Entities:

Entity 1, NterWW12 183 residues - Formula weight is not available

1

GLY

ALA

MET

GLY

HIS

PRO

GLY

MET

HIS

TYR

2

ALA

PRO

MET

GLY

MET

HIS

PRO

MET

GLY

GLN

3

ARG

ALA

ASN

MET

PRO

VAL

PRO

HIS

GLY

4

MET

PRO

GLN

MET

PRO

MET

GLY

5

GLY

PRO

MET

GLY

GLN

MET

PRO

GLY

MET

6

MET

SER

VAL

MET

PRO

GLY

MET

7

SER

HIS

MET

SER

GLN

ALA

SER

MET

GLN

PRO

8

ALA

LEU

PRO

GLY

VAL

ASN

SER

MET

ASP

9

VAL

ALA

GLY

THR

ALA

SER

GLY

ALA

LYS

10

SER

MET

TRP

THR

GLU

HIS

LYS

SER

PRO

ASP

11

GLY

ARG

THR

TYR

ASN

THR

GLU

THR

12

LYS

GLN

SER

THR

TRP

GLU

LYS

PRO

ASP

13

LEU

LYS

THR

PRO

ALA

GLU

GLN

LEU

SER

14

LYS

CYS

PRO

TRP

LYS

GLU

TYR

LYS

SER

ASP

15

SER

GLY

LYS

PRO

TYR

ASN

SER

GLN

16

THR

LYS

GLU

SER

ARG

TRP

ALA

LYS

PRO

LYS

17

GLU

LEU

GLU

ASP

LEU

GLU

GLY

TYR

GLN

ASN

18

THR

ILE

VAL

ALA

GLY

SER

LEU

ILE

THR

LYS

19

SER

ASN

LEU

Samples:

sample_1: PRPF40A, [U-100% 13C; U-100% 15N], 150 uM; sodium phosphate 20 mM; potassium chloride 100 mM; DTT 0.5 mM

sample_conditions_1: ionic strength: 133 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HCC(CO)NH	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN - collection

NMRPipe - processing

CcpNMR - chemical shift assignment

NMR spectrometers:

Bruker AVANCE III 800 MHz