BMRB Entry 52042

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52042

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Title:
AtMed25ACID + DREB2A ABS
Deposition date:
2023-07-15
Original release date:
2023-07-21
Authors:
Theisen, Frederik; Prestel, Andreas; Leurs, Yannick; Kragelund, Birthe
Citation:

Citation: Theisen, Frederik; Prestel, Andreas; Elkjaer, Steffie; Leurs, Yannick; Morffy, Nicholas; Strader, Lucia; O'shea, Charlotte; Teilum, Kaare; Kragelund, Birthe; Skriver, Karen. "Molecular switching in transcription through splicing and proline-isomerization regulates stress responses in plants"  Nat. Comm. 15, 592-592 (2024).
PubMed: 38238333

Assembly members:

Assembly members:
entity_1, polymer, 150 residues, Formula weight is not available
entity_2, polymer, 22 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Thale cress   Taxonomy ID: 3702   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Arabidopsis thaliana

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX 4t1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GSNSSAPNMQLSQPSSGAMQ TSQSKYVKVWEGNLSGQRQG QPVLITRLEGYRSASASDSL AANWPPTMQIVRLISQDHMN NKQYVGKADFLVFRAMSQHG FLGQLQDKKLCAVIQLPSQT LLLSVSDKACRLIGMLFPGD MVVFKPQIPN
entity_2: QDSLSVADYGWPNDVDQSHL DS

Data sets:
Data typeCount
15N chemical shifts135
1H chemical shifts135

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Med251
2DREB2A2

Entities:

Entity 1, Med25 150 residues - Formula weight is not available

Med25ACID 532-680

1   GLYSERASNSERSERALAPROASNMETGLN
2   LEUSERGLNPROSERSERGLYALAMETGLN
3   THRSERGLNSERLYSTYRVALLYSVALTRP
4   GLUGLYASNLEUSERGLYGLNARGGLNGLY
5   GLNPROVALLEUILETHRARGLEUGLUGLY
6   TYRARGSERALASERALASERASPSERLEU
7   ALAALAASNTRPPROPROTHRMETGLNILE
8   VALARGLEUILESERGLNASPHISMETASN
9   ASNLYSGLNTYRVALGLYLYSALAASPPHE
10   LEUVALPHEARGALAMETSERGLNHISGLY
11   PHELEUGLYGLNLEUGLNASPLYSLYSLEU
12   CYSALAVALILEGLNLEUPROSERGLNTHR
13   LEULEULEUSERVALSERASPLYSALACYS
14   ARGLEUILEGLYMETLEUPHEPROGLYASP
15   METVALVALPHELYSPROGLNILEPROASN

Entity 2, DREB2A 22 residues - Formula weight is not available

DREB2A 234-256

1   GLNASPSERLEUSERVALALAASPTYRGLY
2   TRPPROASNASPVALASPGLNSERHISLEU
3   ASPSER

Samples:

sample_1: Med25ACID, [U-100% 13C; U-100% 15N], 500 uM; DREB2A 234-256 2000 uM; Sodium Phosphate buffer 20 mM; NaCl 100 mM; DTT 5 mM

sample_conditions_1: ionic strength: 100 mM; pH: 6.5; pressure: 1 atm; temperature: 298.15 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

CcpNMR - chemical shift assignment

qMDD - processing

NMR spectrometers:

  • Bruker AVANCE III 600 MHz
  • Bruker AVANCE III 750 MHz
  • Bruker AVANCE III 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks