BMRB Entry 52037

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52037

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Title:
Backbone assignment of NFAT1 1-130 S110A
Deposition date:
2023-07-14
Original release date:
2026-03-26
Authors:
Viennet, Thibault; Fischer, Patrick; Arthanari, Haribabu
Citation:

Citation: Viennet, Thibault; Fischer, Patrick; Dempersmier, Jon; Arthanari, Haribabu. "A single phosphorylation site modulates NFAT transactivation"  .

Assembly members:

Assembly members:
entity_1, polymer, 130 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MNAPERQPQPDGGDAPGHEP GGSPQDELDFSILFDYEYLN PNEEEPNAHKVASPPSGPAY PDDVLDYGLKPYSPLASLSG EPPGRFGEPDRVGPQKFLSA AKPAGASGLAPRIEITPSHE LIQAVGPLRM

Data sets:
Data typeCount
13C chemical shifts338
15N chemical shifts104
1H chemical shifts104

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1NFAT1_1-130_S110A1

Entities:

Entity 1, NFAT1_1-130_S110A 130 residues - Formula weight is not available

1   METASNALAPROGLUARGGLNPROGLNPRO
2   ASPGLYGLYASPALAPROGLYHISGLUPRO
3   GLYGLYSERPROGLNASPGLULEUASPPHE
4   SERILELEUPHEASPTYRGLUTYRLEUASN
5   PROASNGLUGLUGLUPROASNALAHISLYS
6   VALALASERPROPROSERGLYPROALATYR
7   PROASPASPVALLEUASPTYRGLYLEULYS
8   PROTYRSERPROLEUALASERLEUSERGLY
9   GLUPROPROGLYARGPHEGLYGLUPROASP
10   ARGVALGLYPROGLNLYSPHELEUSERALA
11   ALALYSPROALAGLYALASERGLYLEUALA
12   PROARGILEGLUILETHRPROSERHISGLU
13   LEUILEGLNALAVALGLYPROLEUARGMET

Samples:

sample_1: NFAT_1-130_S110A, [U-100% 13C; U-100% 15N], 350 uM; MES 50 mM; sodium chloride 150 mM; TCEP 5 mM

sample_conditions_1: ionic strength: 0.2 M; pH: 6.5; pressure: 1 atm; temperature: 288 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)CBsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1

Software:

TOPSPIN - collection

NMRPipe - processing

CcpNMR v2.4.2 - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE III 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks