BMRB Entry 52034
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52034
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NMR-STAR v3 text file.
XML gzip file.
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Citation: Hubner, Julian. "Ded1p RecA1 domain" to be published ., .-..
Assembly members:
entity_1, polymer, 288 residues, Formula weight is not available
Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pETM-11
Entity Sequences (FASTA):
entity_1: GRSGGRWIDGKHVPAPRNEK
AEIAIFGVPEDPNFQSSGIN
FDNYDDIPVDASGKDVPEPI
TEFTSPPLDGLLLENIKLAR
FTKPTPVQKYSVPIVANGRD
LMACAQTGSGKTGGFLFPVL
SESFKTGPSPQPESQGSFYQ
RKAYPTAVIMAPTRELATQI
FDEAKKFTYRSWVKACVVYG
GSPIGNQLREIERGCDLLVA
TPGRLNDLLERGKISLANVK
YLVLDEADRMLDMGFEPQIR
HIVEDCDMTPVGERQTLMFS
ATFPADIQHLARDFLSDYIF
LSVGRVGS
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 420 |
| 15N chemical shifts | 211 |
| 1H chemical shifts | 211 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Ded1p RecA1 domain | 1 |
Entities:
Entity 1, Ded1p RecA1 domain 288 residues - Formula weight is not available
| 1 | GLY | ARG | SER | GLY | GLY | ARG | TRP | ILE | ASP | GLY | ||||
| 2 | LYS | HIS | VAL | PRO | ALA | PRO | ARG | ASN | GLU | LYS | ||||
| 3 | ALA | GLU | ILE | ALA | ILE | PHE | GLY | VAL | PRO | GLU | ||||
| 4 | ASP | PRO | ASN | PHE | GLN | SER | SER | GLY | ILE | ASN | ||||
| 5 | PHE | ASP | ASN | TYR | ASP | ASP | ILE | PRO | VAL | ASP | ||||
| 6 | ALA | SER | GLY | LYS | ASP | VAL | PRO | GLU | PRO | ILE | ||||
| 7 | THR | GLU | PHE | THR | SER | PRO | PRO | LEU | ASP | GLY | ||||
| 8 | LEU | LEU | LEU | GLU | ASN | ILE | LYS | LEU | ALA | ARG | ||||
| 9 | PHE | THR | LYS | PRO | THR | PRO | VAL | GLN | LYS | TYR | ||||
| 10 | SER | VAL | PRO | ILE | VAL | ALA | ASN | GLY | ARG | ASP | ||||
| 11 | LEU | MET | ALA | CYS | ALA | GLN | THR | GLY | SER | GLY | ||||
| 12 | LYS | THR | GLY | GLY | PHE | LEU | PHE | PRO | VAL | LEU | ||||
| 13 | SER | GLU | SER | PHE | LYS | THR | GLY | PRO | SER | PRO | ||||
| 14 | GLN | PRO | GLU | SER | GLN | GLY | SER | PHE | TYR | GLN | ||||
| 15 | ARG | LYS | ALA | TYR | PRO | THR | ALA | VAL | ILE | MET | ||||
| 16 | ALA | PRO | THR | ARG | GLU | LEU | ALA | THR | GLN | ILE | ||||
| 17 | PHE | ASP | GLU | ALA | LYS | LYS | PHE | THR | TYR | ARG | ||||
| 18 | SER | TRP | VAL | LYS | ALA | CYS | VAL | VAL | TYR | GLY | ||||
| 19 | GLY | SER | PRO | ILE | GLY | ASN | GLN | LEU | ARG | GLU | ||||
| 20 | ILE | GLU | ARG | GLY | CYS | ASP | LEU | LEU | VAL | ALA | ||||
| 21 | THR | PRO | GLY | ARG | LEU | ASN | ASP | LEU | LEU | GLU | ||||
| 22 | ARG | GLY | LYS | ILE | SER | LEU | ALA | ASN | VAL | LYS | ||||
| 23 | TYR | LEU | VAL | LEU | ASP | GLU | ALA | ASP | ARG | MET | ||||
| 24 | LEU | ASP | MET | GLY | PHE | GLU | PRO | GLN | ILE | ARG | ||||
| 25 | HIS | ILE | VAL | GLU | ASP | CYS | ASP | MET | THR | PRO | ||||
| 26 | VAL | GLY | GLU | ARG | GLN | THR | LEU | MET | PHE | SER | ||||
| 27 | ALA | THR | PHE | PRO | ALA | ASP | ILE | GLN | HIS | LEU | ||||
| 28 | ALA | ARG | ASP | PHE | LEU | SER | ASP | TYR | ILE | PHE | ||||
| 29 | LEU | SER | VAL | GLY | ARG | VAL | GLY | SER |
Samples:
sample_1: Ded1p N-terminal IDR, [U-13C; U-15N], 900 uM; sodium chloride 325 mM; HEPES 20 mM; DTT 1 mM; sodium azide 0.03%
sample_conditions_1: ionic strength: 0.325 M; pH: 7.3; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-15N TROSY | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CACB | sample_1 | isotropic | sample_conditions_1 |
| 3D C(CO)NH | sample_1 | isotropic | sample_conditions_1 |
Software:
CARA - chemical shift assignment
NMR spectrometers:
- Bruker AVANCE NEO 800 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks