BMRB Entry 52032

Title:
Ded1p C-terminal IDR R540G, R544G, R545G, R550G, R554G, R557G, R571G, R573G, R578G
Deposition date:
2023-07-13
Original release date:
2023-07-21
Authors:
Hubner, Julian
Citation:

Citation: Hubner, Julian. "Ded1p C-terminal IDR R540G, R544G, R545G, R550G, R554G, R557G, R571G, R573G, R578G"  to be published ., .-..

Assembly members:

Assembly members:
entity_1, polymer, 72 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-GB1

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts114
15N chemical shifts57
1H chemical shifts57

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Ded1p C-terminal IDR R540G, R544G, R545G, R550G, R554G, R557G, R571G, R573G, R578G1

Entities:

Entity 1, Ded1p C-terminal IDR R540G, R544G, R545G, R550G, R554G, R557G, R571G, R573G, R578G 72 residues - Formula weight is not available

1   METMETSERALAPROGLYSERGLYSERASN
2   SERGLYGLYGLYGLYPHEGLYGLYASNASN
3   ASNGLYASPTYRGLYLYSALAGLYGLYALA
4   SERALAGLYGLYTRPGLYSERSERGLYSER
5   GLYASPASNSERPHEGLYGLYGLYSERGLY
6   TRPGLYSERASPSERLYSSERSERGLYTRP
7   GLYASNSERGLYGLYSERASNASNSERSER
8   TRPTRP

Samples:

sample_1: Ded1p C-terminal IDR R540G, R544G, R545G, R550G, R554G, R557G, R571G, R573G, R578G, [U-13C; U-15N], 300 uM; sodium chloride 125 mM; HEPES 20 mM; DTT 1 mM; sodium azide 0.03%

sample_conditions_1: ionic strength: 0.125 M; pH: 7.3; pressure: 1 atm; temperature: 277 K

Experiments:

NameSampleSample stateSample conditions
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HN(CO)CACBsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1

Software:

CARA - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE NEO 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks