BMRB Entry 52031

Name: Ded1p C-terminal IDR R540K, R544K, F548G, R545K, R550K, R554K, Y556G, R557K, W567G, R571K, R573K, F577G, R578K, W583G, W592G
Published: 2023-07-21

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52031

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Ded1p C-terminal IDR R540K, R544K, F548G, R545K, R550K, R554K, Y556G, R557K, W567G, R571K, R573K, F577G, R578K, W583G, W592G

Deposition date:

2023-07-13

Original release date:

2023-07-21

Authors:

Hubner, Julian

Citation:

Citation: Hubner, Julian. "Ded1p C-terminal IDR R540K, R544K, F548G, R545K, R550K, R554K, Y556G, R557K, W567G, R571K, R573K, F577G, R578K, W583G, W592G" to be published ., .-..

Assembly members:

Assembly members:
entity_1, polymer, 72 residues, Formula weight is not available

Natural source:

Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-GB1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MMSAPGSKSNSKKGGGGKNN NKDGKKAGGASAGGGGSSKS KDNSGKGGSGGGSDSKSSGG GNSGGSNNSSWW

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	107
15N chemical shifts	55
1H chemical shifts	55

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Ded1p C-terminal IDR R540K, R544K, F548G, R545K, R550K, R554K, Y556G, R557K, W567G, R571K, R573K, F577G, R578K, W583G, W592G	1

Entities:

Entity 1, Ded1p C-terminal IDR R540K, R544K, F548G, R545K, R550K, R554K, Y556G, R557K, W567G, R571K, R573K, F577G, R578K, W583G, W592G 72 residues - Formula weight is not available

1

MET

SER

ALA

PRO

GLY

SER

LYS

SER

ASN

2

SER

LYS

GLY

LYS

ASN

3

ASN

LYS

ASP

GLY

LYS

ALA

GLY

ALA

4

SER

ALA

GLY

SER

LYS

SER

5

LYS

ASP

ASN

SER

GLY

LYS

GLY

SER

GLY

6

GLY

SER

ASP

SER

LYS

SER

GLY

7

GLY

ASN

SER

GLY

SER

ASN

SER

8

TRP

Samples:

sample_1: Ded1p C-terminal IDR R540K, R544K, F548G, R545K, R550K, R554K, Y556G, R557K, W567G, R571K, R573K, F577G, R578K, W583G, W592G, [U-13C; U-15N], 300 uM; sodium chloride 125 mM; HEPES 20 mM; DTT 1 mM; sodium azide 0.03%

sample_conditions_1: ionic strength: 0.125 M; pH: 7.3; pressure: 1 atm; temperature: 277 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
2D 1H-15N TROSY	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
3D HN(CO)CACB	sample_1	isotropic	sample_conditions_1

Software:

CARA - chemical shift assignment

NMR spectrometers:

Bruker AVANCE NEO 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks