BMRB Entry 52026

Name: NMR assignment of the interlinker domain (res. 176 to 246) of the Nucleocapsid Protein of SARS-CoV-2
Published: 2025-06-18

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52026

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

NMR assignment of the interlinker domain (res. 176 to 246) of the Nucleocapsid Protein of SARS-CoV-2

Deposition date:

2023-07-12

Original release date:

2025-06-18

Authors:

Hernandez Gras, Guillem; Martins, Maria; Veloso, Tiago; Lopes, Joao; Castro, Laura; Gomes, Tiago; Cordeiro, Tiago

Citation:

Citation: Hernandez Gras, Guillem; Martins, Maria; Veloso, Tiago; Lopes, Joao; Castro, Laura; Gomes, Tiago; Cordeiro, Tiago. "Dynamic ensembles of SARS-CoV-2 N-protein reveal head-to-head coiled-coil-driven oligomerization and phase separation" Nucleic Acids Res. 53, gkaf502-gkaf502 (2025).
PubMed: 40503686

Assembly members:

Assembly members:
entity_1, polymer, 81 residues, 8400 Da.

Natural source:

Natural source: Common Name: SARS-CoV-2 Taxonomy ID: 2697049 Superkingdom: Viruses Kingdom: not available Genus/species: Betacoronavirus HCoV-SARS

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pHTP8

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GPSRGGSQASSRSSSRSRNS SRNSTPGSSRGTSPARMAGN GGDAALALLLLDRLNQLESK MSGKGQQQQGQTVWSHPQFE K

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	217
15N chemical shifts	76
1H chemical shifts	76

Time Domain Data

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	N3, chain 1	1
2	N3, chain 2	1

Entities:

Entity 1, N3, chain 1 81 residues - 8400 Da.

1

GLY

PRO

SER

ARG

GLY

SER

GLN

ALA

SER

2

SER

ARG

SER

ARG

SER

ARG

ASN

SER

3

SER

ARG

ASN

SER

THR

PRO

GLY

SER

ARG

4

GLY

THR

SER

PRO

ALA

ARG

MET

ALA

GLY

ASN

5

GLY

ASP

ALA

LEU

ALA

LEU

6

LEU

ASP

ARG

LEU

ASN

GLN

LEU

GLU

SER

LYS

7

MET

SER

GLY

LYS

GLY

GLN

GLY

8

GLN

THR

VAL

TRP

SER

HIS

PRO

GLN

PHE

GLU

9

LYS

Samples:

sample_1: N3 domain of the Nucleocapsid Protein of SARS-CoV-2, [U-100% 13C; U-100% 15N], 42 uM; D2O 8%; DSS 20 uM; potassium phosphate 50 mM; sodium chloride 250 mM

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 288 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-15N TROSY	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CO)CACB	sample_1	isotropic	sample_conditions_1
(H)N(CACO)NH	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN v4.2.6 - collection

NMR spectrometers:

Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks