BMRB Entry 52022

Name: Backbone and side chain assignments for RNA recognition motif (RRM) 1 of CP29A from Arabidopsis thaliana
Published: 2024-09-10

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52022

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone and side chain assignments for RNA recognition motif (RRM) 1 of CP29A from Arabidopsis thaliana

Deposition date:

2023-07-10

Original release date:

2024-09-10

Authors:

Kachariya, Nitin; Sattler, Michael; Legen, Julia; Schmitz-Linneweber, Christian

Citation:

Citation: Legen, Julia; Lenzen, Benjamin; Kachariya, Nitin; Feltgen, Stephanie; Gao, Yang; Mergenthal, Simon; Weber, Willi; Klotzsch, Enrico; Zoschke, Reimo; Sattler, Michael; Schmitz-Linneweber, Christian. "A prion-like domain is required for phase separation and chloroplast RNA processing during cold acclimation in Arabidopsis" Plant Cell 36, 2851-2872 (2024).
PubMed: 38723165

Assembly members:

Assembly members:
entity_1, polymer, 83 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Thale cress Taxonomy ID: 3702 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Arabidopsis thaliana

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-M11

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GAMPDLKLFVGNLSFNVDSA QLAQLFESAGNVEMVEVIYD KVTGRSRGFGFVTMSTAAEV EAAAQQFNGYEFEGRPLRVN AGP

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	226
15N chemical shifts	77
1H chemical shifts	461

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	RRM1	1

Entities:

Entity 1, RRM1 83 residues - Formula weight is not available

1

GLY

ALA

MET

PRO

ASP

LEU

LYS

LEU

PHE

VAL

2

GLY

ASN

LEU

SER

PHE

ASN

VAL

ASP

SER

ALA

3

GLN

LEU

ALA

GLN

LEU

PHE

GLU

SER

ALA

GLY

4

ASN

VAL

GLU

MET

VAL

GLU

VAL

ILE

TYR

ASP

5

LYS

VAL

THR

GLY

ARG

SER

ARG

GLY

PHE

GLY

6

PHE

VAL

THR

MET

SER

THR

ALA

GLU

VAL

7

GLU

ALA

GLN

PHE

ASN

GLY

TYR

8

GLU

PHE

GLU

GLY

ARG

PRO

LEU

ARG

VAL

ASN

9

ALA

GLY

PRO

Samples:

sample_1: RRM1 of CP29A, [U-100% 13C; U-100% 15N], 400 uM; RRM1 of CP29A, [U-15N], 400 uM

sample_conditions_1: ionic strength: 50 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1
3D (H)CCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN v3.5 pl 7 - collection

ANALYSIS v2.5 - chemical shift assignment, data analysis

NMR spectrometers:

Bruker AVANCE III 600 MHz
Bruker AVANCE III 800 MHz