BMRB Entry 52012

Name: Chemical shifts of Methanosarcina acetivorans mechanosensitive channel of large conductance(Ma-MscL) in native cellular membranes by solid-state NMR
Published: 2023-07-05

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52012

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Chemical shifts of Methanosarcina acetivorans mechanosensitive channel of large conductance(Ma-MscL) in native cellular membranes by solid-state NMR

Deposition date:

2023-07-03

Original release date:

2023-07-05

Authors:

Zhang, Yan

Citation:

Citation: Zhang, Yan; Gan, Yuefang; Zhao, Weijing; Zhang, Xuning; Zhao, Yongxiang; Xie, Huayong; Yang, Jun. "Membrane Protein Structures in Native Cellular Membranes Revealed by Solid-State NMR Spectroscopy" JACS Au. 3, 3412-3423 (2023).
PubMed: 38155644

Assembly members:

Assembly members:
entity_1, polymer, 101 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Methanosarcina acetivorans Taxonomy ID: 2214 Superkingdom: Archaea Kingdom: not available Genus/species: Methanosarcina acetivorans

Experimental source:

Experimental source: Production method: purified from the natural source

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MGLFSEFKEFLYEYKVIPLA IAFIMGIASTALIKSFVDNI IMPIITPFVPGGGWETATVE LGPIVISWGAFLGELVNFII IAFAVFIIAKKVLQEEKVEK K

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	302
15N chemical shifts	73

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	MaMscL, subunit 1	1
2	MaMscL, subunit 2	1
3	MaMscL, subunit 3	1
4	MaMscL, subunit 4	1
5	MaMscL, subunit 5	1

Entities:

Entity 1, MaMscL, subunit 1 101 residues - Formula weight is not available

1

MET

GLY

LEU

PHE

SER

GLU

PHE

LYS

GLU

PHE

2

LEU

TYR

GLU

TYR

LYS

VAL

ILE

PRO

LEU

ALA

3

ILE

ALA

PHE

ILE

MET

GLY

ILE

ALA

SER

THR

4

ALA

LEU

ILE

LYS

SER

PHE

VAL

ASP

ASN

ILE

5

ILE

MET

PRO

ILE

THR

PRO

PHE

VAL

PRO

6

GLY

TRP

GLU

THR

ALA

THR

VAL

GLU

7

LEU

GLY

PRO

ILE

VAL

ILE

SER

TRP

GLY

ALA

8

PHE

LEU

GLY

GLU

LEU

VAL

ASN

PHE

ILE

9

ILE

ALA

PHE

ALA

VAL

PHE

ILE

ALA

LYS

10

LYS

VAL

LEU

GLN

GLU

LYS

VAL

GLU

LYS

11

LYS

Samples:

sample_1: Inner membranes containing MaMscL, [U-100% 13C; U-100% 15N], 76.9% % w/w; H2O 23.1% % w/w

sample_conditions_1: pH: 8.0; pressure: 101.325 kPa; temperature: 277 K

Experiments:

Name	Sample	Sample state	Sample conditions
NCACX	sample_1	isotropic	sample_conditions_1
NCOCX	sample_1	isotropic	sample_conditions_1
CONCA	sample_1	isotropic	sample_conditions_1

Software:

TALOS+ - data analysis

SPARKY - chemical shift assignment

NMR spectrometers:

Bruker Avance 800 MHz