BMRB Entry 51997

Name: DNAJB6_JD-GF A50V
Published: 2023-10-30

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51997

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

DNAJB6_JD-GF A50V

Deposition date:

2023-06-12

Original release date:

2023-10-30

Authors:

Abayev-Avraham, Meital; Salzberg, Yehuda; Oren-Suissa, Meital; Rosenzweig, Rina

Citation:

Citation: Abayev-Avraham, Meital; Salzberg, Yehuda; Gliksberg, Dar; Oren-Suissa, Meital; Rosenzweig, Rina. "DNAJB6 mutants display toxic gain of function through unregulated interaction with Hsp70 chaperones" Nat. Commun. 14, 7066-7066 (2023).
PubMed: 37923706

Assembly members:

Assembly members:
entity_1, polymer, 108 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-sumo

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MVDYYEVLGVQRHASPEDIK KAYRKLALKWHPDKNPENKE EAERKFKQVVEAYEVLSDAK KRDIYDKYGKEGLNGGGGGG SHFDSPFEFGFTFRNPDDVF REFFGGRD

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	299
15N chemical shifts	99
1H chemical shifts	99

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	DNAJB6 A50V	1

Entities:

Entity 1, DNAJB6 A50V 108 residues - Formula weight is not available

1

MET

VAL

ASP

TYR

GLU

VAL

LEU

GLY

VAL

2

GLN

ARG

HIS

ALA

SER

PRO

GLU

ASP

ILE

LYS

3

LYS

ALA

TYR

ARG

LYS

LEU

ALA

LEU

LYS

TRP

4

HIS

PRO

ASP

LYS

ASN

PRO

GLU

ASN

LYS

GLU

5

GLU

ALA

GLU

ARG

LYS

PHE

LYS

GLN

VAL

6

GLU

ALA

TYR

GLU

VAL

LEU

SER

ASP

ALA

LYS

7

LYS

ARG

ASP

ILE

TYR

ASP

LYS

TYR

GLY

LYS

8

GLU

GLY

LEU

ASN

GLY

9

SER

HIS

PHE

ASP

SER

PRO

PHE

GLU

PHE

GLY

10

PHE

THR

PHE

ARG

ASN

PRO

ASP

VAL

PHE

11

ARG

GLU

PHE

GLY

ARG

ASP

Samples:

sample_1: DNAJB6_JD_GF A50V, [U-100% 13C; U-100% 15N], 2.5 mM

sample_conditions_1: ionic strength: 50 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCACONH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
2D 1H-13C HMQC	sample_1	isotropic	sample_conditions_1

Software:

CcpNMR - chemical shift assignment, peak picking

TOPSPIN - collection

NMRPipe - processing

TOPSPIN - collection

NMR spectrometers:

Bruker AVANCE NEO 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks