BMRB Entry 5199

Name: Assignment of the 1H and 15N resonances of the elicitor protein NIP1
Published: 2003-06-09

Click here to enlarge.

PDB ID: 1kg1
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5199
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

Assignment of the 1H and 15N resonances of the elicitor protein NIP1

Deposition date:

2001-11-02

Original release date:

2003-06-09

Authors:

van't Slot, Klaas; van den Burg, Harrold; Kloks, Cathelijne; Hilbers, Cornelis; Knogge, Wolfgang; Papavoine, Christina

Citation:

Citation: van't Slot, Klaas; van den Burg, Harrold; Kloks, Cathelijne; Hilbers, Cornelis; Knogge, Wolfgang; Papavoine, Christina. "Solution structure of the fungal plant disease resistance-triggering protein NIP1 shows a novel b sheet fold" .

Assembly members:

Assembly members:
Necrosis Inducing Protein 1, polymer, 60 residues, 6434 Da.

Natural source:

Natural source: Common Name: barley leaf scald fungus Taxonomy ID: 38038 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Rhynchosporium secalis

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Necrosis Inducing Protein 1: DRCRYTLCCDGALKAVSACL HESESCLVPGDCCRGKSRLT LCSYGEGGNGFQCPTGYRQC

Data sets:

assigned_chemical_shifts
- nip1.cs
coupling_constants
- nip1_J-couplings

Data type	Count
15N chemical shifts	65
1H chemical shifts	369
coupling constants	35

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	NIP1	1

Entities:

Entity 1, NIP1 60 residues - 6434 Da.

1	ASP	ARG	CYS	ARG	TYR	THR	LEU	CYS	CYS	ASP
2	GLY	ALA	LEU	LYS	ALA	VAL	SER	ALA	CYS	LEU
3	HIS	GLU	SER	GLU	SER	CYS	LEU	VAL	PRO	GLY
4	ASP	CYS	CYS	ARG	GLY	LYS	SER	ARG	LEU	THR
5	LEU	CYS	SER	TYR	GLY	GLU	GLY	GLY	ASN	GLY
6	PHE	GLN	CYS	PRO	THR	GLY	TYR	ARG	GLN	CYS

Samples:

sample-1: Necrosis Inducing Protein 1, [U-15N], 2.0 mM

conditions1: pH: 6.0; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-1H COSY	not available	not available	not available
2D 1H-1H NOESY	not available	not available	not available
2D 1H-1H CLEAN-TOCSY	not available	not available	not available
2D 1H-15N HSQC	not available	not available	not available
2D HMQCJ	not available	not available	not available
3D 1H-1H-15N NOESY	not available	not available	not available
3D 1H-15N-15N NOESY	not available	not available	not available
3D 1H-1H-15N TOCSY	not available	not available	not available
3D HNHA	not available	not available	not available
3D HNHB	not available	not available	not available

Software:

NMRPipe -

XEASY -

NMR spectrometers:

Bruker DMX 600 MHz
Varian INOVA 500 MHz
Varian INOVA 600 MHz

PDB	1KG1
GB	AAA86496 AAS84297 AAS84298 AAS84299 AAS84300

1	ASP	ARG	CYS	ARG	TYR	THR	LEU	CYS	CYS	ASP
2	GLY	ALA	LEU	LYS	ALA	VAL	SER	ALA	CYS	LEU
3	HIS	GLU	SER	GLU	SER	CYS	LEU	VAL	PRO	GLY
4	ASP	CYS	CYS	ARG	GLY	LYS	SER	ARG	LEU	THR
5	LEU	CYS	SER	TYR	GLY	GLU	GLY	GLY	ASN	GLY
6	PHE	GLN	CYS	PRO	THR	GLY	TYR	ARG	GLN	CYS

1	ASP	ARG	CYS	ARG	TYR	THR	LEU	CYS	CYS	ASP
2	GLY	ALA	LEU	LYS	ALA	VAL	SER	ALA	CYS	LEU
3	HIS	GLU	SER	GLU	SER	CYS	LEU	VAL	PRO	GLY
4	ASP	CYS	CYS	ARG	GLY	LYS	SER	ARG	LEU	THR
5	LEU	CYS	SER	TYR	GLY	GLU	GLY	GLY	ASN	GLY
6	PHE	GLN	CYS	PRO	THR	GLY	TYR	ARG	GLN	CYS