BMRB Entry 51975

Name: Chemical shift assignments for the sixth and seventh (HhH)2 repeats from Topoisomerase V
Published: 2023-08-19

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51975

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

Chemical shift assignments for the sixth and seventh (HhH)2 repeats from Topoisomerase V

Deposition date:

2023-05-23

Original release date:

2023-08-19

Authors:

Petersen, Mark; Fang, Rebecca; Majumdar, Ananya; Barrick, Doug

Citation:

Citation: Petersen, Mark; Fang, Rebecca; Majumdar, Ananya; Barrick, Doug. "Stability Islands and the Folding Cooperativity of a Seven-Repeat Array from Topoisomerase V" J. Am. Chem. Soc. 145, 12641-12650 (2023).
PubMed: 37262333

Assembly members:

Assembly members:
entity_1, polymer, 135 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Methanopyrus kandleri Taxonomy ID: 2320 Superkingdom: Archaea Kingdom: not available Genus/species: Methanopyrus kandleri

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pMal

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GGSWGIPKKLREAFDLETAA ELYERYGSLKEIGRRLSYDD LLELGATPKAAAEIKGPEFK FLLNIEGVGPKLAERILEAV DYDLERLASLNPEELAEKVE GLGEELAERVVYAARERVES RRKSGRQERSEEEWK

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1
- assigned_chemical_shifts_2

Data type	Count
13C chemical shifts	369
15N chemical shifts	200
1H chemical shifts	200

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Topoisomerase V repeats FG	1

Entities:

Entity 1, Topoisomerase V repeats FG 135 residues - Formula weight is not available

1

GLY

SER

TRP

GLY

ILE

PRO

LYS

LEU

2

ARG

GLU

ALA

PHE

ASP

LEU

GLU

THR

ALA

3

GLU

LEU

TYR

GLU

ARG

TYR

GLY

SER

LEU

LYS

4

GLU

ILE

GLY

ARG

LEU

SER

TYR

ASP

5

LEU

GLU

LEU

GLY

ALA

THR

PRO

LYS

ALA

6

ALA

GLU

ILE

LYS

GLY

PRO

GLU

PHE

LYS

7

PHE

LEU

ASN

ILE

GLU

GLY

VAL

GLY

PRO

8

LYS

LEU

ALA

GLU

ARG

ILE

LEU

GLU

ALA

VAL

9

ASP

TYR

ASP

LEU

GLU

ARG

LEU

ALA

SER

LEU

10

ASN

PRO

GLU

LEU

ALA

GLU

LYS

VAL

GLU

11

GLY

LEU

GLY

GLU

LEU

ALA

GLU

ARG

VAL

12

VAL

TYR

ALA

ARG

GLU

ARG

VAL

GLU

SER

13

ARG

LYS

SER

GLY

ARG

GLN

GLU

ARG

SER

14

GLU

TRP

LYS

Samples:

sample_1: Topoisomerase V repeat FG, [U-100% 13C; U-100% 15N], 575 uM; sodium phosphate 50 mM; sodium chloride 50 mM

sample_2: Topoisomerase V repeat FG, [U-100% 15N], 825 uM; sodium phosphate 50 mM; sodium chloride 50 mM

sample_conditions_1: pH: 6.4; pressure: 1 atm; temperature: 313 K

sample_conditions_2: pH: 6.4; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N TROSY	sample_2	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
2D 1H-15N TROSY	sample_2	isotropic	sample_conditions_2
3D HN(CA)N	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN - collection

NMRPipe - processing

TALOS-N - data analysis

CARA - chemical shift assignment

CS-Rosetta - structure solution

NMR spectrometers:

Bruker AVANCE II 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks