BMRB Entry 51974

Name: Chemical shift assignments for the seventh (HhH)2 repeat from Topoisomerase V
Published: 2023-08-19

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51974

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Chemical shift assignments for the seventh (HhH)2 repeat from Topoisomerase V

Deposition date:

2023-05-23

Original release date:

2023-08-19

Authors:

Petersen, Mark; Fang, Rebecca; Majumdar, Ananya; Barrick, Doug

Citation:

Citation: Petersen, Mark; Fang, Rebecca; Majumdar, Ananya; Barrick, Doug. "Stability Islands and the Folding Cooperativity of a Seven-Repeat Array from Topoisomerase V" J. Am. Chem. Soc. 145, 12641-12650 (2023).
PubMed: 37262333

Assembly members:

Assembly members:
entity_1, polymer, 84 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Methanopyrus kandleri Taxonomy ID: 2320 Superkingdom: Archaea Kingdom: not available Genus/species: Methanopyrus kandleri

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pMal

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GGSWGPEFKFLLNIEGVGPK LAERILEAVDYDLERLASLN PEELAEKVEGLGEELAERVV YAARERVESRRKSGRQERSE EEWK

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	331
15N chemical shifts	79
1H chemical shifts	489

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Topoisomerase V repeat G	1

Entities:

Entity 1, Topoisomerase V repeat G 84 residues - Formula weight is not available

1

GLY

SER

TRP

GLY

PRO

GLU

PHE

LYS

PHE

2

LEU

ASN

ILE

GLU

GLY

VAL

GLY

PRO

LYS

3

LEU

ALA

GLU

ARG

ILE

LEU

GLU

ALA

VAL

ASP

4

TYR

ASP

LEU

GLU

ARG

LEU

ALA

SER

LEU

ASN

5

PRO

GLU

LEU

ALA

GLU

LYS

VAL

GLU

GLY

6

LEU

GLY

GLU

LEU

ALA

GLU

ARG

VAL

7

TYR

ALA

ARG

GLU

ARG

VAL

GLU

SER

ARG

8

ARG

LYS

SER

GLY

ARG

GLN

GLU

ARG

SER

GLU

9

GLU

TRP

LYS

Samples:

sample_1: Topoisomerase V repeat G, [U-100% 13C; U-100% 15N], 325 uM; sodium phosphate 50 mM; sodium chloride 50 mM

sample_2: Topoisomerase V repeat G, [U-100% 15N], 625 uM; sodium phosphate 50 mM; sodium chloride 50 mM

sample_conditions_1: pH: 6.4; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_2	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D (H)CCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC methyl	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN - collection

NMRPipe - processing

TALOS-N - data analysis

CARA - chemical shift assignment

CS-Rosetta - structure solution

NMR spectrometers:

Bruker AVANCE II 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks