BMRB Entry 51970
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51970
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
All files associated with the entry
Citation: Delaforge, Elise; Elkjaer, Steffie; Due, Amanda; Blackledge, Martin; Skriver, Karen; Kragelund, Birthe. "Allovalent scavenging of activation domains in the transcription factor ANAC013 gears transcriptional regulation" Nucleic Acids Res. ., .-..
Assembly members:
entity_1, polymer, 75 residues, Formula weight is not available
entity_2, polymer, 114 residues, Formula weight is not available
Natural source: Common Name: Thale cress Taxonomy ID: 3702 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Arabidopsis thaliana
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-24b
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 187 |
| 15N chemical shifts | 60 |
| 1H chemical shifts | 60 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | RCD1-RST(499-572) | 1 |
| 2 | ANAC013(161-274) | 2 |
Entities:
Entity 1, RCD1-RST(499-572) 75 residues - Formula weight is not available
M498 is not part of RCD1-RST sequence, residue left from cloning
| 1 | MET | SER | THR | THR | ARG | PRO | LYS | SER | PRO | TRP | ||||
| 2 | MET | PRO | PHE | PRO | THR | LEU | PHE | ALA | ALA | ILE | ||||
| 3 | SER | HIS | LYS | VAL | ALA | GLU | ASN | ASP | MET | LEU | ||||
| 4 | LEU | ILE | ASN | ALA | ASP | TYR | GLN | GLN | LEU | ARG | ||||
| 5 | ASP | LYS | LYS | MET | THR | ARG | ALA | GLU | PHE | VAL | ||||
| 6 | ARG | LYS | LEU | ARG | VAL | ILE | VAL | GLY | ASP | ASP | ||||
| 7 | LEU | LEU | ARG | SER | THR | ILE | THR | THR | LEU | GLN | ||||
| 8 | ASN | GLN | PRO | LYS | SER |
Entity 2, ANAC013(161-274) 114 residues - Formula weight is not available
| 1 | SER | GLY | SER | GLY | PRO | LYS | ASN | GLY | GLU | GLN | ||||
| 2 | TYR | GLY | ALA | PRO | PHE | ILE | GLU | GLU | GLU | TRP | ||||
| 3 | ALA | GLU | ASP | ASP | ASP | ASP | ASP | VAL | ASP | GLU | ||||
| 4 | PRO | ALA | ASN | GLN | LEU | VAL | VAL | SER | ALA | SER | ||||
| 5 | VAL | ASP | ASN | SER | LEU | TRP | GLY | LYS | GLY | LEU | ||||
| 6 | ASN | GLN | SER | GLU | LEU | ASP | ASP | ASN | ASP | ILE | ||||
| 7 | GLU | GLU | LEU | MET | SER | GLN | VAL | ARG | ASP | GLN | ||||
| 8 | SER | GLY | PRO | THR | LEU | GLN | GLN | ASN | GLY | VAL | ||||
| 9 | SER | GLY | LEU | ASN | SER | HIS | VAL | ASP | THR | TYR | ||||
| 10 | ASN | LEU | GLU | ASN | LEU | GLU | GLU | ASP | MET | TYR | ||||
| 11 | LEU | GLU | ILE | ASN | ASP | LEU | MET | GLU | PRO | GLU | ||||
| 12 | PRO | GLU | PRO | THR |
Samples:
sample_1: RCD1-RST(499-572), [U-13C; U-15N], 200 uM; ANAC013 161-274 200 uM
sample_conditions_1: ionic strength: 0.17 M; pH: 7.4; pressure: 1 atm; temperature: 293 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CACB | sample_1 | isotropic | sample_conditions_1 |
Software:
MARS - chemical shift assignment
qMDD - processing
NMRFAM-SPARKY - peak picking
NMR spectrometers:
- Bruker AVANCE III 750 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks