BMRB Entry 51959

Assembly:

Entities:

Entity 1, sigma1.1 79 residues - 9379.02 Da.

This protein sequence is a fragment (2-72) of sA primary transcription factor. The initial methionine at position 1 is N-terminally cleaved. Sequence numbering in our case begins with 1 ALA, 2 ASP, ... Residues 72-73 represent non-native residues inserted because of the restriction enzyme (XhoI) used for cloning. Residues 74-79 represent a non-native affinity tag.

Samples:

sample_1: sigma1.1, [U-99% 13C; U-99% 15N], 1 mM; D2O 10%; H2O 90%; sodium phosphate 20 mM; sodium chloride 10 mM; sodium azide 3 mM

sample_2: sigma1.1, [U-99% 15N], 0.8 mM; H2O 100%; sodium phosphate 20 mM; sodium chloride 10 mM; sodium azide 3 mM

sample_3: sigma1.1, [U-15N; U-2H; 99%] + specific labeling of 13C pro-S methyls, 0.8 mM; H2O 100%; sodium phosphate 20 mM; sodium chloride 10 mM; sodium azide 3 mM

sample_4: sigma1.1, [U-15N; U-2H; 99%], 0.8 mM; H2O 100%; sodium phosphate 20 mM; sodium chloride 10 mM; sodium azide 3 mM

sample_5: sigma1.1, selectively labeled C-alpha positions except for Val, Leu, Ile,, 0.8 mM; D2O 100%; sodium phosphate 20 mM; sodium chloride 10 mM; sodium azide 3 mM

sample_conditions_1: pH: 6.6; pressure: 1 atm; temperature: 298.15 K

Experiments:

Software:

GLOVE v2.4 - chemical shift calculation from CPMG dispersion profiles

ChemEx v2021.4.0.dev6 - chemical shift calculation from CPMG and CEST profiles

NMRFAM-SPARKY - chemical shift assignment

NMR spectrometers:

• Bruker DRX 950 MHz
• Bruker DRX 850 MHz
• Bruker DRX 700 MHz
• Bruker DRX 600 MHz