BMRB Entry 51957

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51957

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Title:
Human eIF4B C-terminal region
Deposition date:
2023-05-07
Original release date:
2023-05-13
Authors:
Mondal, Somnath; Rousseau, Sabrina; Aznauryan, Mikayel; Mackereth, Cameron
Citation:

Citation: Mondal, Somnath; Rousseau, Sabrina; Talenton, Vincent; Thiam, Cheikh Ahmadou Bamba; Aznauryan, Mikayel; Mackereth, Cameron. "Backbone resonance assignments of the C-terminal region of human translation initiation factor eIF4B"  Biomol. NMR Assign. 17, 199-203 (2023).
PubMed: 37368134

Assembly members:

Assembly members:
entity_1, polymer, 281 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: 2B-T

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GCKLNLKPRSTPKEDDSSAS TSQSTRAASIFGGAKPVDTA AREREVEERLQKEQEKLQRQ LDEPKLERRPRERHPSWRSE ETQERERSRTGSESSQTGTS TTSSRNARRRESEKSLENET LNKEEDCHSPTSKPPKPDQP LKVMPAPPPKENAWVKRSSN PPARSQSSDTEQQSPTSGGG KVAPAQPSEEGPGRKDENKV DGMNAPKGQTGNSSRGPGDG GNRDHWKESDRKDGKKDQDS RSAPEPKKPEENPASKFSSA SKYAALSVDGEDENEGEDYA E

Data sets:
Data typeCount
13C chemical shifts534
15N chemical shifts267
1H chemical shifts243

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1eIF4B-CTR1

Entities:

Entity 1, eIF4B-CTR 281 residues - Formula weight is not available

Gly-Cys- was added at the N-terminus

1   GLYCYSLYSLEUASNLEULYSPROARGSER
2   THRPROLYSGLUASPASPSERSERALASER
3   THRSERGLNSERTHRARGALAALASERILE
4   PHEGLYGLYALALYSPROVALASPTHRALA
5   ALAARGGLUARGGLUVALGLUGLUARGLEU
6   GLNLYSGLUGLNGLULYSLEUGLNARGGLN
7   LEUASPGLUPROLYSLEUGLUARGARGPRO
8   ARGGLUARGHISPROSERTRPARGSERGLU
9   GLUTHRGLNGLUARGGLUARGSERARGTHR
10   GLYSERGLUSERSERGLNTHRGLYTHRSER
11   THRTHRSERSERARGASNALAARGARGARG
12   GLUSERGLULYSSERLEUGLUASNGLUTHR
13   LEUASNLYSGLUGLUASPCYSHISSERPRO
14   THRSERLYSPROPROLYSPROASPGLNPRO
15   LEULYSVALMETPROALAPROPROPROLYS
16   GLUASNALATRPVALLYSARGSERSERASN
17   PROPROALAARGSERGLNSERSERASPTHR
18   GLUGLNGLNSERPROTHRSERGLYGLYGLY
19   LYSVALALAPROALAGLNPROSERGLUGLU
20   GLYPROGLYARGLYSASPGLUASNLYSVAL
21   ASPGLYMETASNALAPROLYSGLYGLNTHR
22   GLYASNSERSERARGGLYPROGLYASPGLY
23   GLYASNARGASPHISTRPLYSGLUSERASP
24   ARGLYSASPGLYLYSLYSASPGLNASPSER
25   ARGSERALAPROGLUPROLYSLYSPROGLU
26   GLUASNPROALASERLYSPHESERSERALA
27   SERLYSTYRALAALALEUSERVALASPGLY
28   GLUASPGLUASNGLUGLYGLUASPTYRALA
29   GLU

Samples:

sample_1: eIF4B-CTR, [U-100% 13C; U-100% 15N], 150 uM; sodium phosphate 20 mM; sodium chloride 150 mM; DTT 2 mM

sample_conditions_1: ionic strength: 170 mM; pH: 7.0; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
1D 1Hsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HA(CACO)NHsample_1isotropicsample_conditions_1
3D HNHAsample_1isotropicsample_conditions_1
3D N(COCA)NNHsample_1isotropicsample_conditions_1
3D HACANsample_1isotropicsample_conditions_1
3D HACA(CO)Nsample_1isotropicsample_conditions_1

Software:

TOPSPIN - collection

NMRPipe - processing

NMRFAM-SPARKY - chemical shift assignment, data analysis

NMR spectrometers:

  • Bruker AVANCE NEO 700 MHz

Related Database Links:

UNP P23588
AlphaFold Q8WYK5

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks