BMRB Entry 51953

Name: Human eIF4B second half of C-terminal region
Published: 2023-05-13

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51953

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Human eIF4B second half of C-terminal region

Deposition date:

2023-05-04

Original release date:

2023-05-13

Authors:

Mondal, Somnath; Rousseau, Sabrina; Aznauryan, Mikayel; Mackereth, Cameron

Citation:

Citation: Mondal, Somnath; Rousseau, Sabrina; Talenton, Vincent; Thiam, Cheikh Ahmadou Bamba; Aznauryan, Mikayel; Mackereth, Cameron. "Backbone resonance assignments of the C-terminal region of human translation initiation factor eIF4B" Biomol. NMR Assign. 17, 199-203 (2023).
PubMed: 37368134

Assembly members:

Assembly members:
entity_1, polymer, 155 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: 2B-T

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GHSPTSKPPKPDQPLKVMPA PPPKENAWVKRSSNPPARSQ SSDTEQQSPTSGGGKVAPAQ PSEEGPGRKDENKVDGMNAP KGQTGNSSRGPGDGGNRDHW KESDRKDGKKDQDSRSAPEP KKPEENPASKFSSASKYAAL SVDGEDENEGEDYAE

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	431
15N chemical shifts	152
1H chemical shifts	296

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	eIF4B-CTRC	1

Entities:

Entity 1, eIF4B-CTRC 155 residues - Formula weight is not available

Gly- was added at the N-terminus

1

GLY

HIS

SER

PRO

THR

SER

LYS

PRO

LYS

2

PRO

ASP

GLN

PRO

LEU

LYS

VAL

MET

PRO

ALA

3

PRO

LYS

GLU

ASN

ALA

TRP

VAL

LYS

4

ARG

SER

ASN

PRO

ALA

ARG

SER

GLN

5

SER

ASP

THR

GLU

GLN

SER

PRO

THR

6

SER

GLY

LYS

VAL

ALA

PRO

ALA

GLN

7

PRO

SER

GLU

GLY

PRO

GLY

ARG

LYS

ASP

8

GLU

ASN

LYS

VAL

ASP

GLY

MET

ASN

ALA

PRO

9

LYS

GLY

GLN

THR

GLY

ASN

SER

ARG

GLY

10

PRO

GLY

ASP

GLY

ASN

ARG

ASP

HIS

TRP

11

LYS

GLU

SER

ASP

ARG

LYS

ASP

GLY

LYS

12

ASP

GLN

ASP

SER

ARG

SER

ALA

PRO

GLU

PRO

13

LYS

PRO

GLU

ASN

PRO

ALA

SER

LYS

14

PHE

SER

ALA

SER

LYS

TYR

ALA

LEU

15

SER

VAL

ASP

GLY

GLU

ASP

GLU

ASN

GLU

GLY

16

GLU

ASP

TYR

ALA

GLU

Samples:

sample_1: CTRC, [U-100% 13C; U-100% 15N], 668 uM; sodium phosphate 20 mM; sodium chloride 150 mM; DTT 2 mM

sample_conditions_1: ionic strength: 170 mM; pH: 7.0; pressure: 1 atm; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
1D 1H	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HA(CACO)NH	sample_1	isotropic	sample_conditions_1
3D HNHA	sample_1	isotropic	sample_conditions_1
3D N(COCA)NNH	sample_1	isotropic	sample_conditions_1
3D HACAN	sample_1	isotropic	sample_conditions_1
3D HACA(CO)N	sample_1	isotropic	sample_conditions_1
3D H(NCOCA)NNH	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN - collection

NMRPipe - processing

NMRFAM-SPARKY - chemical shift assignment, data analysis

NMR spectrometers:

Bruker AVANCE NEO 700 MHz

UNP	P23588
AlphaFold	Q8WYK5