BMRB Entry 51940

Name: Backbone Assignment of Human Polymerase Theta MOTIF 2 phosphorylated by PLK1
Published: 2023-10-03

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51940

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

Backbone Assignment of Human Polymerase Theta MOTIF 2 phosphorylated by PLK1

Deposition date:

2023-04-26

Original release date:

2023-10-03

Authors:

Zinn-Justin, Sophie; Miron, Simona; Boeffard-Dosierre, Liza; Airs-Loeuilliette, Julie

Citation:

Citation: Gelot, Camille; Kovacs, Marton Tibor; Miron, Simona; Mylne, Emilie; Haan, Alexis; Boeffard-Dosierre, Liza; Ghouil, Rania; Popova, Tatiana; Dingli, Florent; Loew, Damarys; Guirouilh-Barbat, Josee; Del Nery, Elaine; Zinn-Justin, Sophie; Ceccaldi, Raphael. "Polth is phosphorylated by PLK1 to repair double-strand breaks in mitosis" Nature 621, 415-422 (2023).
PubMed: 37674080

Assembly members:

Assembly members:
entity_1, polymer, 121 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-22b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: ENQDTHQQLTQXNDEXIIFS EMDXVQMVEALDNVDIFPVQ EKNHZVVSPRALELXDPVLD EHHQGDQDGGDQDERAEKSK LTGTRQNHXFIWSGAXFDLS PGLQRILDKVSSPLENEKLK S

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	340
15N chemical shifts	111
1H chemical shifts	111

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Human Polymerase Theta MOTIF 2 phosphorylated by PLK1	1

Entities:

Entity 1, Human Polymerase Theta MOTIF 2 phosphorylated by PLK1 121 residues - Formula weight is not available

1

GLU

ASN

GLN

ASP

THR

HIS

GLN

LEU

THR

2

GLN

SEP

ASN

ASP

GLU

SEP

ILE

PHE

SER

3

GLU

MET

ASP

SEP

VAL

GLN

MET

VAL

GLU

ALA

4

LEU

ASP

ASN

VAL

ASP

ILE

PHE

PRO

VAL

GLN

5

GLU

LYS

ASN

HIS

TPO

VAL

SER

PRO

ARG

6

ALA

LEU

GLU

LEU

SEP

ASP

PRO

VAL

LEU

ASP

7

GLU

HIS

GLN

GLY

ASP

GLN

ASP

GLY

8

ASP

GLN

ASP

GLU

ARG

ALA

GLU

LYS

SER

LYS

9

LEU

THR

GLY

THR

ARG

GLN

ASN

HIS

SEP

PHE

10

ILE

TRP

SER

GLY

ALA

SEP

PHE

ASP

LEU

SER

11

PRO

GLY

LEU

GLN

ARG

ILE

LEU

ASP

LYS

VAL

12

SER

PRO

LEU

GLU

ASN

GLU

LYS

LEU

LYS

13

SER

Samples:

sample_1: Human Polymerase Theta MOTIF 2 phosphorylated by PLK1, [U-95% 13C; U-95% 15N], 300 uM

sample_conditions_1: ionic strength: 0.05 M; pH: 7; pressure: 1 atm; temperature: 283 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACO	sample_1	isotropic	sample_conditions_1
3D HNCANNH	sample_1	isotropic	sample_conditions_1

Software:

CcpNMR - chemical shift assignment

NMR spectrometers:

Bruker AVANCE NEO 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks