BMRB Entry 5193

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PDB ID: 1jzc
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5193
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

The solution structure of the mutant 5'AUG3' triloop in the RNA promoter region of the brome mosaic virus genomic (+)-RNA

Deposition date:

2001-10-30

Original release date:

2003-01-06

Authors:

Kim, C.-H.; Kao, C.

Citation:

Citation: Kim, C.-H.; Kao, C.. "A mutant viral RNA promoter with an altered conformation retains efficient recognition by a viral RNA replicase through a solution-exposed adenine." RNA 7, 1476-1485 (2001).
PubMed: 11680852

Assembly members:

Assembly members:
5'-R(*GP*GP*UP*GP*CP*AP*UP*GP*GP*CP*AP*CP*C)-3', polymer, 13 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Brome mosaic virus Taxonomy ID: 12302 Superkingdom: Viruses Kingdom: not available Genus/species: Bromovirus Brome mosaic virus

Experimental source:

Experimental source: Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
5'-R(*GP*GP*UP*GP*CP*AP*UP*GP*GP*CP*AP*CP*C)-3': GGUGCAUGGCACC

Data sets:

assigned_chemical_shifts
- chemical_shift_set_1
- chemical_shift_set_2

Data type	Count
1H chemical shifts	18

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	5'-R(GPGPUPGPCPAPUPGPGPCPAPCP*C)-3'	1

Entities:

Entity 1, 5'-R(*GP*GP*UP*GP*CP*AP*UP*GP*GP*CP*AP*CP*C)-3' 13 residues - Formula weight is not available

1

G

U

G

C

A

U

G

C

2

A

C

Samples:

sample_1: 5'-R(*GP*GP*UP*GP*CP*AP*UP*GP*GP*CP*AP*CP*C)-3' 2 mM; sodium phosphate 10 mM; NaCl 10 mM

sample_2: 5'-R(*GP*GP*UP*GP*CP*AP*UP*GP*GP*CP*AP*CP*C)-3' 2 mM; sodium phosphate 10 mM; NaCl 10 mM; D2O 99.96%

sample_cond_1: ionic strength: 10 mM; pH: 6.5; pressure: 1 atm; temperature: 293 K

sample_cond_2: ionic strength: 10 mM; pH: 6.5; pressure: 1 atm; temperature: 283 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D NOESY	not available	not available	not available
DQF-COSY	not available	not available	not available

Software:

X-PLOR v3.1 - structure solution

xwinnmr - collection

UXNMR - collection

FELIX - data analysis

NMR spectrometers:

Bruker AMX 600 MHz