BMRB Entry 51928

Name: Solid-state NMR data for 40-residue Arctic (E22G) Beta-Amyloid Fibril
Published: 2024-09-27

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51928

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solid-state NMR data for 40-residue Arctic (E22G) Beta-Amyloid Fibril

Deposition date:

2023-04-18

Original release date:

2024-09-27

Authors:

Tehrani, Mohammad; Isamu, Matsuda; Matsunaga, Tatsuya; Ishii, Yoshitaka

Citation:

Citation: Tehrani, Mohammad Jafar; Matsuda, Isamu; Yamagata, Atsushi; Kodama, Yu; Matsunaga, Tatsuya; Sato, Mayuko; Toyooka, Kiminori; McElheny, Dan; Kobayashi, Naohiro; Shirouzu, Mikako; Ishii, Yoshitaka. "E22G Ab40 fibril structure and kinetics illuminate how Ab40 rather than Ab42 triggers familial Alzheimer's" Nat. Commun. 15, 7045-7045 (2024).
PubMed: 39147751

Assembly members:

Assembly members:
entity_1, polymer, 40 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: DAEFRHDSGYEVHHQKLVFF AGDVGSNKGAIIGLMVGGVV

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	95

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	E22G Abeta40	1

Entities:

Entity 1, E22G Abeta40 40 residues - Formula weight is not available

1	ASP	ALA	GLU	PHE	ARG	HIS	ASP	SER	GLY	TYR
2	GLU	VAL	HIS	HIS	GLN	LYS	LEU	VAL	PHE	PHE
3	ALA	GLY	ASP	VAL	GLY	SER	ASN	LYS	GLY	ALA
4	ILE	ILE	GLY	LEU	MET	VAL	GLY	GLY	VAL	VAL

Samples:

sample_1: Amyloid fibril, 13C, 15N labeled at selected sites, N/A N/A

sample_conditions_1: ionic strength: 10 mM; pH: 7.4; pressure: 1 atm; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 13C-13C DARR	sample_1	isotropic	sample_conditions_1
1H-detected (H)CH Hector	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe - data analysis

NMR spectrometers:

Bruker Avance 400 MHz
JEOL ECZR 900 MHz

1	ASP	ALA	GLU	PHE	ARG	HIS	ASP	SER	GLY	TYR
2	GLU	VAL	HIS	HIS	GLN	LYS	LEU	VAL	PHE	PHE
3	ALA	GLY	ASP	VAL	GLY	SER	ASN	LYS	GLY	ALA
4	ILE	ILE	GLY	LEU	MET	VAL	GLY	GLY	VAL	VAL

1	ASP	ALA	GLU	PHE	ARG	HIS	ASP	SER	GLY	TYR
2	GLU	VAL	HIS	HIS	GLN	LYS	LEU	VAL	PHE	PHE
3	ALA	GLY	ASP	VAL	GLY	SER	ASN	LYS	GLY	ALA
4	ILE	ILE	GLY	LEU	MET	VAL	GLY	GLY	VAL	VAL

1	ASP	ALA	GLU	PHE	ARG	HIS	ASP	SER	GLY	TYR
2	GLU	VAL	HIS	HIS	GLN	LYS	LEU	VAL	PHE	PHE
3	ALA	GLY	ASP	VAL	GLY	SER	ASN	LYS	GLY	ALA
4	ILE	ILE	GLY	LEU	MET	VAL	GLY	GLY	VAL	VAL