BMRB Entry 51907

Name: Backbone resonance assignment of a stabilized neurotensin receptor bound to neurotensin
Published: 2023-05-07

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51907

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone resonance assignment of a stabilized neurotensin receptor bound to neurotensin

Deposition date:

2023-04-14

Original release date:

2023-05-07

Authors:

Mohamadi, Mariam; Hagn, Franz

Citation:

Citation: Mohamadi, Mariam; Goricanec, David; Wagner, Gerhard; Hagn, Franz. "NMR sample optimization and backbone assignment of a stabilized neurotensin receptor" J. Struc. Biol. 215, 107970-107970 (2023).
PubMed: 37142193

Assembly members:

Assembly members:
entity_1, polymer, 350 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Rat Taxonomy ID: 10116 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Rattus norvegicus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pEP-HTGH4dIC3B G50-G391

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GPNSDLDVNTDIYSKVLVTA IYLALFVVGTVGNGVTLFTL ARKKSLQSLQSRVDYYLGSL ALSDLLILLFALPVDLYNFI WVHHPWAFGDAGCKGYYFLR EACTYATALNVVSLSVELYL AICHPFKAKTLMSRSRTKKF ISAIWLASALLAIPMLFTMG LQNLSGDGTHPGGLVCTPIV DTATLRVVIQLNTFMSFLFP MLVASILNTVAARRLTVMVH QAAEQGRVCTVGTHNGLEHS TFNMTIEPGRVQALRRGVLV LRAVVIAFVVCWLPYHVRRL MFVYISDEQWTTALFDFYHY FYMLSNALVYVSAAINPILY NLVSANFRQVFLSTLACLCP GTRELEVLFQ

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	337
15N chemical shifts	108
1H chemical shifts	108

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	NTR1	1

Entities:

Entity 1, NTR1 350 residues - Formula weight is not available

1	GLY	PRO	ASN	SER	ASP	LEU	ASP	VAL	ASN	THR
2	ASP	ILE	TYR	SER	LYS	VAL	LEU	VAL	THR	ALA
3	ILE	TYR	LEU	ALA	LEU	PHE	VAL	VAL	GLY	THR
4	VAL	GLY	ASN	GLY	VAL	THR	LEU	PHE	THR	LEU
5	ALA	ARG	LYS	LYS	SER	LEU	GLN	SER	LEU	GLN
6	SER	ARG	VAL	ASP	TYR	TYR	LEU	GLY	SER	LEU
7	ALA	LEU	SER	ASP	LEU	LEU	ILE	LEU	LEU	PHE
8	ALA	LEU	PRO	VAL	ASP	LEU	TYR	ASN	PHE	ILE
9	TRP	VAL	HIS	HIS	PRO	TRP	ALA	PHE	GLY	ASP
10	ALA	GLY	CYS	LYS	GLY	TYR	TYR	PHE	LEU	ARG
11	GLU	ALA	CYS	THR	TYR	ALA	THR	ALA	LEU	ASN
12	VAL	VAL	SER	LEU	SER	VAL	GLU	LEU	TYR	LEU
13	ALA	ILE	CYS	HIS	PRO	PHE	LYS	ALA	LYS	THR
14	LEU	MET	SER	ARG	SER	ARG	THR	LYS	LYS	PHE
15	ILE	SER	ALA	ILE	TRP	LEU	ALA	SER	ALA	LEU
16	LEU	ALA	ILE	PRO	MET	LEU	PHE	THR	MET	GLY
17	LEU	GLN	ASN	LEU	SER	GLY	ASP	GLY	THR	HIS
18	PRO	GLY	GLY	LEU	VAL	CYS	THR	PRO	ILE	VAL
19	ASP	THR	ALA	THR	LEU	ARG	VAL	VAL	ILE	GLN
20	LEU	ASN	THR	PHE	MET	SER	PHE	LEU	PHE	PRO
21	MET	LEU	VAL	ALA	SER	ILE	LEU	ASN	THR	VAL
22	ALA	ALA	ARG	ARG	LEU	THR	VAL	MET	VAL	HIS
23	GLN	ALA	ALA	GLU	GLN	GLY	ARG	VAL	CYS	THR
24	VAL	GLY	THR	HIS	ASN	GLY	LEU	GLU	HIS	SER
25	THR	PHE	ASN	MET	THR	ILE	GLU	PRO	GLY	ARG
26	VAL	GLN	ALA	LEU	ARG	ARG	GLY	VAL	LEU	VAL
27	LEU	ARG	ALA	VAL	VAL	ILE	ALA	PHE	VAL	VAL
28	CYS	TRP	LEU	PRO	TYR	HIS	VAL	ARG	ARG	LEU
29	MET	PHE	VAL	TYR	ILE	SER	ASP	GLU	GLN	TRP
30	THR	THR	ALA	LEU	PHE	ASP	PHE	TYR	HIS	TYR
31	PHE	TYR	MET	LEU	SER	ASN	ALA	LEU	VAL	TYR
32	VAL	SER	ALA	ALA	ILE	ASN	PRO	ILE	LEU	TYR
33	ASN	LEU	VAL	SER	ALA	ASN	PHE	ARG	GLN	VAL
34	PHE	LEU	SER	THR	LEU	ALA	CYS	LEU	CYS	PRO
35	GLY	THR	ARG	GLU	LEU	GLU	VAL	LEU	PHE	GLN

Samples:

sample_1: NTR1 monomer, [U-13C; U-15N; U-2H], 400 uM

sample_conditions_1: ionic strength: 0.1 M; pH: 6.5; pressure: 1 atm; temperature: 315 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N TROSY	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D 15N-separated NOESY	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN v4.0 - collection, processing

NMRPipe - processing

istHMS - processing

NMRFAM-SPARKY - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

Bruker AVANCE III 800 MHz

1	GLY	PRO	ASN	SER	ASP	LEU	ASP	VAL	ASN	THR
2	ASP	ILE	TYR	SER	LYS	VAL	LEU	VAL	THR	ALA
3	ILE	TYR	LEU	ALA	LEU	PHE	VAL	VAL	GLY	THR
4	VAL	GLY	ASN	GLY	VAL	THR	LEU	PHE	THR	LEU
5	ALA	ARG	LYS	LYS	SER	LEU	GLN	SER	LEU	GLN
6	SER	ARG	VAL	ASP	TYR	TYR	LEU	GLY	SER	LEU
7	ALA	LEU	SER	ASP	LEU	LEU	ILE	LEU	LEU	PHE
8	ALA	LEU	PRO	VAL	ASP	LEU	TYR	ASN	PHE	ILE
9	TRP	VAL	HIS	HIS	PRO	TRP	ALA	PHE	GLY	ASP
10	ALA	GLY	CYS	LYS	GLY	TYR	TYR	PHE	LEU	ARG
11	GLU	ALA	CYS	THR	TYR	ALA	THR	ALA	LEU	ASN
12	VAL	VAL	SER	LEU	SER	VAL	GLU	LEU	TYR	LEU
13	ALA	ILE	CYS	HIS	PRO	PHE	LYS	ALA	LYS	THR
14	LEU	MET	SER	ARG	SER	ARG	THR	LYS	LYS	PHE
15	ILE	SER	ALA	ILE	TRP	LEU	ALA	SER	ALA	LEU
16	LEU	ALA	ILE	PRO	MET	LEU	PHE	THR	MET	GLY
17	LEU	GLN	ASN	LEU	SER	GLY	ASP	GLY	THR	HIS
18	PRO	GLY	GLY	LEU	VAL	CYS	THR	PRO	ILE	VAL
19	ASP	THR	ALA	THR	LEU	ARG	VAL	VAL	ILE	GLN
20	LEU	ASN	THR	PHE	MET	SER	PHE	LEU	PHE	PRO
21	MET	LEU	VAL	ALA	SER	ILE	LEU	ASN	THR	VAL
22	ALA	ALA	ARG	ARG	LEU	THR	VAL	MET	VAL	HIS
23	GLN	ALA	ALA	GLU	GLN	GLY	ARG	VAL	CYS	THR
24	VAL	GLY	THR	HIS	ASN	GLY	LEU	GLU	HIS	SER
25	THR	PHE	ASN	MET	THR	ILE	GLU	PRO	GLY	ARG
26	VAL	GLN	ALA	LEU	ARG	ARG	GLY	VAL	LEU	VAL
27	LEU	ARG	ALA	VAL	VAL	ILE	ALA	PHE	VAL	VAL
28	CYS	TRP	LEU	PRO	TYR	HIS	VAL	ARG	ARG	LEU
29	MET	PHE	VAL	TYR	ILE	SER	ASP	GLU	GLN	TRP
30	THR	THR	ALA	LEU	PHE	ASP	PHE	TYR	HIS	TYR
31	PHE	TYR	MET	LEU	SER	ASN	ALA	LEU	VAL	TYR
32	VAL	SER	ALA	ALA	ILE	ASN	PRO	ILE	LEU	TYR
33	ASN	LEU	VAL	SER	ALA	ASN	PHE	ARG	GLN	VAL
34	PHE	LEU	SER	THR	LEU	ALA	CYS	LEU	CYS	PRO
35	GLY	THR	ARG	GLU	LEU	GLU	VAL	LEU	PHE	GLN

1	GLY	PRO	ASN	SER	ASP	LEU	ASP	VAL	ASN	THR
2	ASP	ILE	TYR	SER	LYS	VAL	LEU	VAL	THR	ALA
3	ILE	TYR	LEU	ALA	LEU	PHE	VAL	VAL	GLY	THR
4	VAL	GLY	ASN	GLY	VAL	THR	LEU	PHE	THR	LEU
5	ALA	ARG	LYS	LYS	SER	LEU	GLN	SER	LEU	GLN
6	SER	ARG	VAL	ASP	TYR	TYR	LEU	GLY	SER	LEU
7	ALA	LEU	SER	ASP	LEU	LEU	ILE	LEU	LEU	PHE
8	ALA	LEU	PRO	VAL	ASP	LEU	TYR	ASN	PHE	ILE
9	TRP	VAL	HIS	HIS	PRO	TRP	ALA	PHE	GLY	ASP
10	ALA	GLY	CYS	LYS	GLY	TYR	TYR	PHE	LEU	ARG
11	GLU	ALA	CYS	THR	TYR	ALA	THR	ALA	LEU	ASN
12	VAL	VAL	SER	LEU	SER	VAL	GLU	LEU	TYR	LEU
13	ALA	ILE	CYS	HIS	PRO	PHE	LYS	ALA	LYS	THR
14	LEU	MET	SER	ARG	SER	ARG	THR	LYS	LYS	PHE
15	ILE	SER	ALA	ILE	TRP	LEU	ALA	SER	ALA	LEU
16	LEU	ALA	ILE	PRO	MET	LEU	PHE	THR	MET	GLY
17	LEU	GLN	ASN	LEU	SER	GLY	ASP	GLY	THR	HIS
18	PRO	GLY	GLY	LEU	VAL	CYS	THR	PRO	ILE	VAL
19	ASP	THR	ALA	THR	LEU	ARG	VAL	VAL	ILE	GLN
20	LEU	ASN	THR	PHE	MET	SER	PHE	LEU	PHE	PRO
21	MET	LEU	VAL	ALA	SER	ILE	LEU	ASN	THR	VAL
22	ALA	ALA	ARG	ARG	LEU	THR	VAL	MET	VAL	HIS
23	GLN	ALA	ALA	GLU	GLN	GLY	ARG	VAL	CYS	THR
24	VAL	GLY	THR	HIS	ASN	GLY	LEU	GLU	HIS	SER
25	THR	PHE	ASN	MET	THR	ILE	GLU	PRO	GLY	ARG
26	VAL	GLN	ALA	LEU	ARG	ARG	GLY	VAL	LEU	VAL
27	LEU	ARG	ALA	VAL	VAL	ILE	ALA	PHE	VAL	VAL
28	CYS	TRP	LEU	PRO	TYR	HIS	VAL	ARG	ARG	LEU
29	MET	PHE	VAL	TYR	ILE	SER	ASP	GLU	GLN	TRP
30	THR	THR	ALA	LEU	PHE	ASP	PHE	TYR	HIS	TYR
31	PHE	TYR	MET	LEU	SER	ASN	ALA	LEU	VAL	TYR
32	VAL	SER	ALA	ALA	ILE	ASN	PRO	ILE	LEU	TYR
33	ASN	LEU	VAL	SER	ALA	ASN	PHE	ARG	GLN	VAL
34	PHE	LEU	SER	THR	LEU	ALA	CYS	LEU	CYS	PRO
35	GLY	THR	ARG	GLU	LEU	GLU	VAL	LEU	PHE	GLN