BMRB Entry 51904

Name: Modifications to the SR-Rich Region of the SARS-CoV-2 Nucleocapsid Regulate Self-Association and Attenuate RNA Interactions
Published: 2024-09-28

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51904

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Modifications to the SR-Rich Region of the SARS-CoV-2 Nucleocapsid Regulate Self-Association and Attenuate RNA Interactions

Deposition date:

2023-04-13

Original release date:

2024-09-28

Authors:

Reardon, Patrick; Stuwe, Hannah; Yu, Zhen; Shah, Sahana; Hughes, Kaitlyn; Barbar, Elisar

Citation:

Citation: Stuwe, Hannah; Reardon, Patrick; Yu, Zhen; Shah, Sahana; Hughes, Kaitlyn; Barbar, Elisar. "Phosphorylation in the Ser/Arg-rich region of the nucleocapsid of SARS-CoV-2 regulates phase separation by inhibiting self-association of a distant helix" J. Biol. Chem. 300, 107354-107354 (2024).
PubMed: 38718862

Assembly members:

Assembly members:
entity_1, polymer, 80 residues, Formula weight is not available

Natural source:

Natural source: Common Name: SARS-CoV-2 Taxonomy ID: 2697049 Superkingdom: Viruses Kingdom: not available Genus/species: Betacoronavirus HCoV-SARS

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pRBC SUMO

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: SGSRGGSQASSRSSSRSRNS SRNSTPGSSRGTSPARMAGN GGDAALALLLLDRLNQLESK MSGKGQQQQGQTGTENLYFQ

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	198
15N chemical shifts	70
1H chemical shifts	70

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	N145-245	1

Entities:

Entity 1, N145-245 80 residues - Formula weight is not available

1	SER	GLY	SER	ARG	GLY	GLY	SER	GLN	ALA	SER
2	SER	ARG	SER	SER	SER	ARG	SER	ARG	ASN	SER
3	SER	ARG	ASN	SER	THR	PRO	GLY	SER	SER	ARG
4	GLY	THR	SER	PRO	ALA	ARG	MET	ALA	GLY	ASN
5	GLY	GLY	ASP	ALA	ALA	LEU	ALA	LEU	LEU	LEU
6	LEU	ASP	ARG	LEU	ASN	GLN	LEU	GLU	SER	LYS
7	MET	SER	GLY	LYS	GLY	GLN	GLN	GLN	GLN	GLY
8	GLN	THR	GLY	THR	GLU	ASN	LEU	TYR	PHE	GLN

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1

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SPARKY: Backbone or all simulated peaks

BMRB Entry 51904

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

1	SER	GLY	SER	ARG	GLY	GLY	SER	GLN	ALA	SER
2	SER	ARG	SER	SER	SER	ARG	SER	ARG	ASN	SER
3	SER	ARG	ASN	SER	THR	PRO	GLY	SER	SER	ARG
4	GLY	THR	SER	PRO	ALA	ARG	MET	ALA	GLY	ASN
5	GLY	GLY	ASP	ALA	ALA	LEU	ALA	LEU	LEU	LEU
6	LEU	ASP	ARG	LEU	ASN	GLN	LEU	GLU	SER	LYS
7	MET	SER	GLY	LYS	GLY	GLN	GLN	GLN	GLN	GLY
8	GLN	THR	GLY	THR	GLU	ASN	LEU	TYR	PHE	GLN

1	SER	GLY	SER	ARG	GLY	GLY	SER	GLN	ALA	SER
2	SER	ARG	SER	SER	SER	ARG	SER	ARG	ASN	SER
3	SER	ARG	ASN	SER	THR	PRO	GLY	SER	SER	ARG
4	GLY	THR	SER	PRO	ALA	ARG	MET	ALA	GLY	ASN
5	GLY	GLY	ASP	ALA	ALA	LEU	ALA	LEU	LEU	LEU
6	LEU	ASP	ARG	LEU	ASN	GLN	LEU	GLU	SER	LYS
7	MET	SER	GLY	LYS	GLY	GLN	GLN	GLN	GLN	GLY
8	GLN	THR	GLY	THR	GLU	ASN	LEU	TYR	PHE	GLN

1	SER	GLY	SER	ARG	GLY	GLY	SER	GLN	ALA	SER
2	SER	ARG	SER	SER	SER	ARG	SER	ARG	ASN	SER
3	SER	ARG	ASN	SER	THR	PRO	GLY	SER	SER	ARG
4	GLY	THR	SER	PRO	ALA	ARG	MET	ALA	GLY	ASN
5	GLY	GLY	ASP	ALA	ALA	LEU	ALA	LEU	LEU	LEU
6	LEU	ASP	ARG	LEU	ASN	GLN	LEU	GLU	SER	LYS
7	MET	SER	GLY	LYS	GLY	GLN	GLN	GLN	GLN	GLY
8	GLN	THR	GLY	THR	GLU	ASN	LEU	TYR	PHE	GLN