BMRB Entry 51904

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51904

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Title:
Modifications to the SR-Rich Region of the SARS-CoV-2 Nucleocapsid Regulate Self-Association and Attenuate RNA Interactions
Deposition date:
2023-04-13
Original release date:
2024-09-28
Authors:
Reardon, Patrick; Stuwe, Hannah; Yu, Zhen; Shah, Sahana; Hughes, Kaitlyn; Barbar, Elisar
Citation:

Citation: Stuwe, Hannah; Reardon, Patrick; Yu, Zhen; Shah, Sahana; Hughes, Kaitlyn; Barbar, Elisar. "Phosphorylation in the Ser/Arg-rich region of the nucleocapsid of SARS-CoV-2 regulates phase separation by inhibiting self-association of a distant helix"  J. Biol. Chem. 300, 107354-107354 (2024).
PubMed: 38718862

Assembly members:

Assembly members:
entity_1, polymer, 80 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: SARS-CoV-2   Taxonomy ID: 2697049   Superkingdom: Viruses   Kingdom: not available   Genus/species: Betacoronavirus HCoV-SARS

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pRBC SUMO

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: SGSRGGSQASSRSSSRSRNS SRNSTPGSSRGTSPARMAGN GGDAALALLLLDRLNQLESK MSGKGQQQQGQTGTENLYFQ

Data sets:
Data typeCount
13C chemical shifts198
15N chemical shifts70
1H chemical shifts70

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1N145-2451

Entities:

Entity 1, N145-245 80 residues - Formula weight is not available

1   SERGLYSERARGGLYGLYSERGLNALASER
2   SERARGSERSERSERARGSERARGASNSER
3   SERARGASNSERTHRPROGLYSERSERARG
4   GLYTHRSERPROALAARGMETALAGLYASN
5   GLYGLYASPALAALALEUALALEULEULEU
6   LEUASPARGLEUASNGLNLEUGLUSERLYS
7   METSERGLYLYSGLYGLNGLNGLNGLNGLY
8   GLNTHRGLYTHRGLUASNLEUTYRPHEGLN

Samples:

sample_1: N145-245, [U-99% 13C; U-99% 15N], .15 mM; sodium phosphate 50 mM; sodium chloride 150 mM

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 283 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1

Software:

TOPSPIN - collection

NMRPipe - processing

NMRViewJ - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE III HD 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks