BMRB Entry 51901

Title:
Tst2
Deposition date:
2023-04-09
Original release date:
2023-05-10
Authors:
Elnahriry, Khaled; Wai, Dorothy; Norton, Raymond
Citation:

Citation: Elnahriry, Khaled; Wai, Dorothy; Ashwood, Lauren; Naseem, Muhammad Umair; Szanto, Tibor; Guo, Shaodong; Panyi, Gyorgy; Prentis, Peter; Norton, Raymond. "Structural and functional characterisation of Tst2, a novel TRPV1 inhibitory peptide from the Australian sea anemone Telmatactis stephensoni"  Biochim. Biophys. Acta Proteins Proteom. 1872, 140952-140952 (2023).
PubMed: 37640250

Assembly members:

Assembly members:
entity_1, polymer, 39 residues, 4022.7 Da.

Natural source:

Natural source:   Common Name: Telmatactis stephensoni   Taxonomy ID: 2835637   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Telmatactis stephensoni

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET21a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GCKGQDAPCSKSKDCCGEAS MCSKGADGKKTCMIDKLWG

Data sets:
Data typeCount
1H chemical shifts247

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Tst21

Entities:

Entity 1, Tst2 39 residues - 4022.7 Da.

1   GLYCYSLYSGLYGLNASPALAPROCYSSER
2   LYSSERLYSASPCYSCYSGLYGLUALASER
3   METCYSSERLYSGLYALAASPGLYLYSLYS
4   THRCYSMETILEASPLYSLEUTRPGLY

Samples:

sample_1: Tst2, [U-13C; U-15N], 550 uM

sample_conditions_1: ionic strength: 0.55 mM; pH: 4.7; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
3D 15N-separated NOESYsample_1isotropicsample_conditions_1

Software:

TOPSPIN v3.6.3 - collection, processing

SPARKY v3.134 - chemical shift assignment, data analysis, peak picking

NMRPipe - processing

CYANA v3.98.13 - structure solution

X-PLOR NIH v2.48 - refinement

NMR spectrometers:

  • Bruker AVANCE III 600 MHz