BMRB Entry 51901

Name: Tst2
Published: 2023-05-10

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51901

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Tst2

Deposition date:

2023-04-09

Original release date:

2023-05-10

Authors:

Elnahriry, Khaled; Wai, Dorothy; Norton, Raymond

Citation:

Citation: Elnahriry, Khaled; Wai, Dorothy; Ashwood, Lauren; Naseem, Muhammad Umair; Szanto, Tibor; Guo, Shaodong; Panyi, Gyorgy; Prentis, Peter; Norton, Raymond. "Structural and functional characterisation of Tst2, a novel TRPV1 inhibitory peptide from the Australian sea anemone Telmatactis stephensoni" Biochim. Biophys. Acta Proteins Proteom. 1872, 140952-140952 (2023).
PubMed: 37640250

Assembly members:

Assembly members:
entity_1, polymer, 39 residues, 4022.7 Da.

Natural source:

Natural source: Common Name: Telmatactis stephensoni Taxonomy ID: 2835637 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Telmatactis stephensoni

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET21a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GCKGQDAPCSKSKDCCGEAS MCSKGADGKKTCMIDKLWG

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
1H chemical shifts	247

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Tst2	1

Entities:

Entity 1, Tst2 39 residues - 4022.7 Da.

1

GLY

CYS

LYS

GLY

GLN

ASP

ALA

PRO

CYS

SER

2

LYS

SER

LYS

ASP

CYS

GLY

GLU

ALA

SER

3

MET

CYS

SER

LYS

GLY

ALA

ASP

GLY

LYS

4

THR

CYS

MET

ILE

ASP

LYS

LEU

TRP

GLY

Samples:

sample_1: Tst2, [U-13C; U-15N], 550 uM

sample_conditions_1: ionic strength: 0.55 mM; pH: 4.7; pressure: 1 atm; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1
3D 15N-separated NOESY	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN v3.6.3 - collection, processing

SPARKY v3.134 - chemical shift assignment, data analysis, peak picking

NMRPipe - processing

CYANA v3.98.13 - structure solution

X-PLOR NIH v2.48 - refinement

NMR spectrometers:

Bruker AVANCE III 600 MHz