BMRB Entry 51898

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51898

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Title:
DCC N29P1
Deposition date:
2023-04-04
Original release date:
2025-10-08
Authors:
Paukovich, Natasia; Nowling, Nathan; Henen, Morkos; Vogeli, Beat; Filbin, Megan
Citation:

Citation: Paukovich, Natasia; Spear, Elizabeth; MacFadden, Andrea; Nowling, Nathan; Henen, Morkos; Vogeli, Beat; Filbin, Megan. "Intrinsically disordered cytoplasmic tail in netrin receptor DCC binds the large ribosomal subunit to inhibit translation"  J. Biol. Chem. ., .-. (2025).
PubMed: 40975167

Assembly members:

Assembly members:
entity_1, polymer, 47 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-28a(+)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: CTRRSSAQQRKKRATHSAGK RKGSQKDLRPPDLWIHHEEM EMKNIEK

Data sets:
Data typeCount
13C chemical shifts135
15N chemical shifts44
1H chemical shifts44

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1DCC N29P11

Entities:

Entity 1, DCC N29P1 47 residues - Formula weight is not available

1   CYSTHRARGARGSERSERALAGLNGLNARG
2   LYSLYSARGALATHRHISSERALAGLYLYS
3   ARGLYSGLYSERGLNLYSASPLEUARGPRO
4   PROASPLEUTRPILEHISHISGLUGLUMET
5   GLUMETLYSASNILEGLULYS

Samples:

sample_1: DCC N29P1, [U-100% 13C; U-100% 15N], 125 uM; Na-Phosphate Buffer 200 mM; NaCl 100 mM; DTT 2 mM

sample_conditions_1: ionic strength: 100 mM; pH: 6.4; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HNNsample_1isotropicsample_conditions_1

Software:

CcpNMR v2.4.2 - chemical shift assignment, peak picking

NMR spectrometers:

  • Varian INOVA 900 MHz
  • Bruker AVANCE NEO 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks