BMRB Entry 51892

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51892

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
1H, 15N and 13C backbone assignments for human PARP-1 BRCT domain
Deposition date:
2023-03-31
Original release date:
2023-06-19
Authors:
Yang, Ji-Chun; Wu, Wing-Fung; Neuhaus, David
Citation:

Citation: Suskiewicz, Marcin; Munnur, Deeksha; Stromland, Oyvind; Yang, Ji-Chun; Easton, Laura; Chatrin, Chatrin; Zhu, Kang; Baretic, Domagoj; Goffinont, Stephane; Schuller, Marion; Wu, Wing-Fung; Elkins, Jonathan; Ahel, Dragana; Sanyal, Sumana; Neuhaus, David; Ahel, Ivan. "Updated protein domain annotation of the PARP protein family sheds new light on biological function"  Nucleic Acids Res. 51, 8217-8236 (2023).
PubMed: 37326024

Assembly members:

Assembly members:
entity_1, polymer, 146 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a-lip

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GNSSASADKPLSNMKILTLG KLSRNKDEVKAMIEKLGGKL TGTANKASLCISTKKEVEKM NKKMEEVKEANIRVVSEDFL QDVSASTKSLQELFLAHILS PWGAEVKAEPVEVVAPRGKS GAALSKKSKGQVKEEGINKS EKRMKL

Data sets:
Data typeCount
13C chemical shifts221
15N chemical shifts138
1H chemical shifts244

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PARP1 BRCT domain1

Entities:

Entity 1, PARP1 BRCT domain 146 residues - Formula weight is not available

The first three residues Gly-Asn-Ser as shown above are a cloning artefact. The native sequence of PARP-1 BRCT starts at Ser 383 and runs to Leu 525.

1   GLYASNSERSERALASERALAASPLYSPRO
2   LEUSERASNMETLYSILELEUTHRLEUGLY
3   LYSLEUSERARGASNLYSASPGLUVALLYS
4   ALAMETILEGLULYSLEUGLYGLYLYSLEU
5   THRGLYTHRALAASNLYSALASERLEUCYS
6   ILESERTHRLYSLYSGLUVALGLULYSMET
7   ASNLYSLYSMETGLUGLUVALLYSGLUALA
8   ASNILEARGVALVALSERGLUASPPHELEU
9   GLNASPVALSERALASERTHRLYSSERLEU
10   GLNGLULEUPHELEUALAHISILELEUSER
11   PROTRPGLYALAGLUVALLYSALAGLUPRO
12   VALGLUVALVALALAPROARGGLYLYSSER
13   GLYALAALALEUSERLYSLYSSERLYSGLY
14   GLNVALLYSGLUGLUGLYILEASNLYSSER
15   GLULYSARGMETLYSLEU

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks