BMRB Entry 51890

Name: Assignment of human Dynein Light intermediate chain 2 (LIC2)
Published: 2023-07-28

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51890

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

Assignment of human Dynein Light intermediate chain 2 (LIC2)

Deposition date:

2023-03-29

Original release date:

2023-07-28

Authors:

Henen, Morkos; Vogeli, Beat

Citation:

Citation: Henen, Morkos; Paukovich, Natasia; Prekeris, Rytis; Vogeli, Beat. "Solution NMR Backbone Assignment of the C-Terminal Region of Human Dynein Light Intermediate Chain 2 (LIC2-C) Unveils Structural Resemblance with Its Homologue LIC1-C" Magnetochemistry 9, 166-166 (2023).
PubMed: 37476506

Assembly members:

Assembly members:
entity_1, polymer, 132 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX-6p-1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GPWATPTRASESPARGPSGS PRTQGRGGPASVPSSSPGTS VKKPDPNIKNNAASEGVLAS FFNSLLSKKTGSPGSPGAGG VQSTAKKSGQKTVLSNVQEE LDRMTRKPDSMVTNSSTENE AGSGSGHHHHHH

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	189
15N chemical shifts	101
1H chemical shifts	101

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	LIC2	1

Entities:

Entity 1, LIC2 132 residues - Formula weight is not available

1

GLY

PRO

TRP

ALA

THR

PRO

THR

ARG

ALA

SER

2

GLU

SER

PRO

ALA

ARG

GLY

PRO

SER

GLY

SER

3

PRO

ARG

THR

GLN

GLY

ARG

GLY

PRO

ALA

4

SER

VAL

PRO

SER

PRO

GLY

THR

SER

5

VAL

LYS

PRO

ASP

PRO

ASN

ILE

LYS

ASN

6

ASN

ALA

SER

GLU

GLY

VAL

LEU

ALA

SER

7

PHE

ASN

SER

LEU

SER

LYS

THR

8

GLY

SER

PRO

GLY

SER

PRO

GLY

ALA

GLY

9

VAL

GLN

SER

THR

ALA

LYS

SER

GLY

GLN

10

LYS

THR

VAL

LEU

SER

ASN

VAL

GLN

GLU

11

LEU

ASP

ARG

MET

THR

ARG

LYS

PRO

ASP

SER

12

MET

VAL

THR

ASN

SER

THR

GLU

ASN

GLU

13

ALA

GLY

SER

GLY

SER

GLY

HIS

14

HIS

Samples:

sample_1: LIC2, [U-100% 13C; U-100% 15N], 840 uM; NaCl 150 mM

sample_conditions_1: ionic strength: 150 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCACONH	sample_1	isotropic	sample_conditions_1
3D HNN	sample_1	isotropic	sample_conditions_1

Software:

CcpNMR - chemical shift assignment, data analysis

TOPSPIN - collection

NMRFAM-SPARKY - data analysis

NMRPipe - processing

NMR spectrometers:

Bruker AVANCE NEO 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks