BMRB Entry 51889

Name: Larimichthys crocea IFNd
Published: 2024-04-03

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51889

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Larimichthys crocea IFNd

Deposition date:

2023-03-29

Original release date:

2024-04-03

Authors:

Chen, Jingjie

Citation:

Citation: Chen, Jingjie. "1H and 15N Assigned Chemical Shifts for Larimichthys crocea interferon d" J. Immunol. ., .-..

Assembly members:

Assembly members:
entity_1, polymer, 185 residues, Formula weight is not available

Natural source:

Natural source: Common Name: large yellow croaker Taxonomy ID: 215358 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Larimichthys crocea

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MLSRILFVCLSLSLYSAGSS LSCRWVDHKFRQHSETSLDL LNTMANNSTNSTEDAQVEDT VAFPNDLYSQASKASAEDKL HFTVQVLEEAAALFEEDHSN ASWEENTVENFVNVVNQQAD GLRSCTGSHGHKKKNKKLHM YFKRLSSHVLKKMSHSAEAW ELIRKEIRTHLMRADQLVSS LRNTN

Data sets:

spectral_peak_list
- spectral_peak_list_1

Data type

Count

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	interferon	1

Entities:

Entity 1, interferon 185 residues - Formula weight is not available

1

MET

LEU

SER

ARG

ILE

LEU

PHE

VAL

CYS

LEU

2

SER

LEU

SER

LEU

TYR

SER

ALA

GLY

SER

3

LEU

SER

CYS

ARG

TRP

VAL

ASP

HIS

LYS

PHE

4

ARG

GLN

HIS

SER

GLU

THR

SER

LEU

ASP

LEU

5

LEU

ASN

THR

MET

ALA

ASN

SER

THR

ASN

6

SER

THR

GLU

ASP

ALA

GLN

VAL

GLU

ASP

THR

7

VAL

ALA

PHE

PRO

ASN

ASP

LEU

TYR

SER

GLN

8

ALA

SER

LYS

ALA

SER

ALA

GLU

ASP

LYS

LEU

9

HIS

PHE

THR

VAL

GLN

VAL

LEU

GLU

ALA

10

ALA

LEU

PHE

GLU

ASP

HIS

SER

ASN

11

ALA

SER

TRP

GLU

ASN

THR

VAL

GLU

ASN

12

PHE

VAL

ASN

VAL

ASN

GLN

ALA

ASP

13

GLY

LEU

ARG

SER

CYS

THR

GLY

SER

HIS

GLY

14

HIS

LYS

ASN

LYS

LEU

HIS

MET

15

TYR

PHE

LYS

ARG

LEU

SER

HIS

VAL

LEU

16

LYS

MET

SER

HIS

SER

ALA

GLU

ALA

TRP

17

GLU

LEU

ILE

ARG

LYS

GLU

ILE

ARG

THR

HIS

18

LEU

MET

ARG

ALA

ASP

GLN

LEU

VAL

SER

19

LEU

ARG

ASN

THR

ASN

Samples:

sample_1: interferon, [U-95% 13C; U-95% 15N], 500 mM; NaCl 150 mM; Tris-HCl 50 mM

sample_conditions_1: pH: 7.5; temperature: 298.15 K

Experiments:

Name	Sample	Sample state	Sample conditions
1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1

Software:

SPARKY - chemical shift assignment, collection

NMR spectrometers:

Bruker DMX 800 MHz