BMRB Entry 51888
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51888
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NMR-STAR v3 text file.
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Citation: Heber, Simone; McClintock, Mark; Simon, Bernd; Mehtab, Eve; Lapouge, Karine; Hennig, Janosch; Bullock, Simon; Ephrussi, Anne. "Tropomyosin 1-I/C coordinates kinesin-1 and dynein motors during oskar mRNA transport" Nat. Struct. Mol. Biol. 31, 476-488 (2024).
PubMed: 38297086
Assembly members:
entity_1, polymer, 122 residues, Formula weight is not available
Natural source: Common Name: fruit fly Taxonomy ID: 7227 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Drosophila melanogaster
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pETM11
Entity Sequences (FASTA):
entity_1: MSFLENNLDQLTKVHKQLVR
DNADLRCELPKLEKRLRCTM
ERVKALETALKEAKEGAMRD
RKRYQYEVDRIKEAVRQKHL
GRRGPQAQIAKPIRSGQGAI
AIRGGGAVGGPSPLAQVNPV
NS
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 109 |
| 15N chemical shifts | 33 |
| 1H chemical shifts | 152 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Khc_855-975 | 1 |
Entities:
Entity 1, Khc_855-975 122 residues - Formula weight is not available
| 1 | MET | SER | PHE | LEU | GLU | ASN | ASN | LEU | ASP | GLN | ||||
| 2 | LEU | THR | LYS | VAL | HIS | LYS | GLN | LEU | VAL | ARG | ||||
| 3 | ASP | ASN | ALA | ASP | LEU | ARG | CYS | GLU | LEU | PRO | ||||
| 4 | LYS | LEU | GLU | LYS | ARG | LEU | ARG | CYS | THR | MET | ||||
| 5 | GLU | ARG | VAL | LYS | ALA | LEU | GLU | THR | ALA | LEU | ||||
| 6 | LYS | GLU | ALA | LYS | GLU | GLY | ALA | MET | ARG | ASP | ||||
| 7 | ARG | LYS | ARG | TYR | GLN | TYR | GLU | VAL | ASP | ARG | ||||
| 8 | ILE | LYS | GLU | ALA | VAL | ARG | GLN | LYS | HIS | LEU | ||||
| 9 | GLY | ARG | ARG | GLY | PRO | GLN | ALA | GLN | ILE | ALA | ||||
| 10 | LYS | PRO | ILE | ARG | SER | GLY | GLN | GLY | ALA | ILE | ||||
| 11 | ALA | ILE | ARG | GLY | GLY | GLY | ALA | VAL | GLY | GLY | ||||
| 12 | PRO | SER | PRO | LEU | ALA | GLN | VAL | ASN | PRO | VAL | ||||
| 13 | ASN | SER |
Samples:
sample_1: Khc_855-975, [U-100% 13C; U-100% 15N], 180 uM
sample_conditions_1: ionic strength: 150 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HMQC | sample_1 | isotropic | sample_conditions_1 |
| 3D H(CCO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D C(CO)NH | sample_1 | isotropic | sample_conditions_1 |
Software:
CARA - chemical shift assignment
NMR spectrometers:
- Bruker AVANCE III 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks