BMRB Entry 51882

Name: MASTL-phosphorylated ARPP19
Published: 2023-03-23

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51882

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

MASTL-phosphorylated ARPP19

Deposition date:

2023-03-20

Original release date:

2023-03-23

Authors:

Vos, Margaret; Peti, Wolfgang; Page, Rebecca

Citation:

Citation: Padi, Sathish; Vos, Margaret; Godek, Rachel; Fuller, James; Kruse, Thomas; Hein, Jamin; Nilsson, Jakob; Kelker, Matthew; Page, Rebecca; Peti, Wolfgang. "Cryo-EM structures of PP2A:B55-FAM122A and PP2A:B55-ARPP19" Nature 625, 195-203 (2024).
PubMed: 38123684

Assembly members:

Assembly members:
entity_1, polymer, 114 residues, 12517 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pTHMT

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GHMSAEVPEAASAEEQKEME DKVTSPEKAEEAKLKARYPH LGQKPGGSDFLRKRLQKGQK YFDSGDYNMAKAKMKNKQLP TAAPDKTEVTGDHIPTPQDL PQRKPSLVASKLAG

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	275
15N chemical shifts	96
1H chemical shifts	96

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	MASTL-phosphorylated ARPP19	1

Entities:

Entity 1, MASTL-phosphorylated ARPP19 114 residues - 12517 Da.

cAMP-regulated phosphoprotein 19, 112 amino acids starting from "MSA", S62 and S104 are phosphorylated.

1

GLY

HIS

MET

SER

ALA

GLU

VAL

PRO

GLU

ALA

2

ALA

SER

ALA

GLU

GLN

LYS

GLU

MET

GLU

3

ASP

LYS

VAL

THR

SER

PRO

GLU

LYS

ALA

GLU

4

GLU

ALA

LYS

LEU

LYS

ALA

ARG

TYR

PRO

HIS

5

LEU

GLY

GLN

LYS

PRO

GLY

SER

ASP

PHE

6

LEU

ARG

LYS

ARG

LEU

GLN

LYS

GLY

GLN

LYS

7

TYR

PHE

ASP

SEP

GLY

ASP

TYR

ASN

MET

ALA

8

LYS

ALA

LYS

MET

LYS

ASN

LYS

GLN

LEU

PRO

9

THR

ALA

PRO

ASP

LYS

THR

GLU

VAL

THR

10

GLY

ASP

HIS

ILE

PRO

THR

PRO

GLN

ASP

LEU

11

PRO

GLN

ARG

LYS

PRO

SEP

LEU

VAL

ALA

SER

12

LYS

LEU

ALA

GLY

Samples:

sample_1: MASTL-phosphorylated ARPP19, [U-99% 15N], 80 uM; MASTL-phosphorylated ARPP19, [U-99% 13C; U-99% 15N], 80 uM

sample_conditions_1: ionic strength: 150 mM; pH: 6.3; pressure: 1 atm; temperature: 283 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN vTopspin 4.1.1 - collection, processing

CARA - chemical shift assignment, peak picking

NMR spectrometers:

Bruker AVANCE NEO 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks