BMRB Entry 51877
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51877
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NMR-STAR v3 text file.
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Citation: Pipercevic, Joka; Kohl, Bastian; Gerasimaite, Ruta; Comte-Miserez, Veronique; Hostachy, Sarah; Muntener, Thomas; Agustoni, Elia; Jessen, Henning Jacob; Fiedler, Dorothea; Mayer, Andreas; Hiller, Sebastian. "Inositol pyrophosphates activate the vacuolar transport chaperone complex in yeast by disrupting a homotypic SPX domain interaction" Nat. Commun. 14, 2645-2645 (2023).
PubMed: 37156835
Assembly members:
entity_1, polymer, 207 residues, Formula weight is not available
entity_IHP, non-polymer, 660.035 Da.
Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: modified pET
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 365 |
| 15N chemical shifts | 189 |
| 1H chemical shifts | 189 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Vtc2(1-201) Monomer | 1 |
| 2 | inositol hexaphosphate | 2 |
Entities:
Entity 1, Vtc2(1-201) Monomer 207 residues - Formula weight is not available
| 1 | MET | LEU | PHE | GLY | VAL | LYS | LEU | ALA | ASN | GLU | ||||
| 2 | VAL | TYR | PRO | PRO | TRP | LYS | GLY | SER | TYR | ILE | ||||
| 3 | ASN | TYR | GLU | GLY | LEU | LYS | LYS | PHE | LEU | LYS | ||||
| 4 | GLU | ASP | SER | VAL | LYS | ASP | GLY | SER | ASN | ASP | ||||
| 5 | LYS | LYS | ALA | ARG | TRP | ASP | ASP | SER | ASP | GLU | ||||
| 6 | SER | LYS | PHE | VAL | GLU | GLU | LEU | ASP | LYS | GLU | ||||
| 7 | LEU | GLU | LYS | VAL | TYR | GLY | PHE | GLN | LEU | LYS | ||||
| 8 | LYS | TYR | ASN | ASN | LEU | MET | GLU | ARG | LEU | SER | ||||
| 9 | HIS | LEU | GLU | LYS | GLN | THR | ASP | THR | GLU | ALA | ||||
| 10 | ALA | ILE | LYS | ALA | LEU | ASP | ALA | ASP | ALA | PHE | ||||
| 11 | GLN | ARG | VAL | LEU | GLU | GLU | LEU | LEU | SER | GLU | ||||
| 12 | SER | THR | GLU | LEU | ASP | ASN | PHE | LYS | ARG | LEU | ||||
| 13 | ASN | PHE | THR | GLY | PHE | ALA | LYS | ILE | VAL | LYS | ||||
| 14 | LYS | HIS | ASP | LYS | LEU | TYR | PRO | LYS | TYR | PRO | ||||
| 15 | SER | VAL | LYS | SER | LEU | LEU | GLU | VAL | ARG | LEU | ||||
| 16 | LYS | GLU | LEU | PRO | SER | HIS | SER | GLU | GLU | TYR | ||||
| 17 | SER | PRO | LEU | LEU | TYR | ARG | ILE | SER | PHE | LEU | ||||
| 18 | TYR | ASN | ILE | LEU | ARG | SER | ASN | PHE | ASN | THR | ||||
| 19 | ALA | SER | GLU | PRO | LEU | ALA | SER | ALA | SER | LYS | ||||
| 20 | PHE | SER | SER | ILE | VAL | SER | ASN | ASP | ILE | ASP | ||||
| 21 | MET | GLU | ASN | LEU | TYR | PHE | GLN |
Entity 2, inositol hexaphosphate - C6 H18 O24 P6 - 660.035 Da.
| 1 | IHP |
Samples:
sample_1: Vtc2(1-201), [U-13C; U-15N; U-2H], 500 uM; D2O 5%; NaCl 250 mM; EDTA 0.5 mM; TCEP 0.5 mM; HEPES 25 mM; Inositol hexaphosphate 10 eq
sample_conditions_1: pH: 7.0; pressure: 1 atm; temperature: 300 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1D 1H | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-15N TROSY | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
Software:
CARA - data analysis
PROSA - processing
XEASY - data analysis
TOPSPIN - collection
NMR spectrometers:
- Bruker AVANCE III 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks