BMRB Entry 51871

Name: 1H, 13C, and 15N backbone NMR resonance assignments of TANGO1 (30-139) from Drosophila melanogaster
Published: 2023-03-08

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51871

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C, and 15N backbone NMR resonance assignments of TANGO1 (30-139) from Drosophila melanogaster

Deposition date:

2023-03-07

Original release date:

2023-03-08

Authors:

Arnolds, Oliver; Stoll, Raphael

Citation:

Citation: Arnolds, Oliver; Stoll, Raphael. "Characterization of a fold in TANGO1 evolved from SH3 domains for the export of bulky cargos" Nat. Commun. 14, 2273-2273 (2023).
PubMed: 37080980

Assembly members:

Assembly members:
entity_1, polymer, 111 residues, 12115.1615 Da.

Natural source:

Natural source: Common Name: fruit fly Taxonomy ID: 7227 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Drosophila melanogaster

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pQE40

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MTLTWAATLSDKRLCADPKC EQIISMGIAKITYAIGGEGL ISFKINSPIRVLSKSAGSNM QLWGVDINGRRGYANKDFIM EKKILVRDKDLLYEVPVVGP GSPVQSVETPV

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	327
15N chemical shifts	108
1H chemical shifts	316

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	TANGO DROME	1

Entities:

Entity 1, TANGO DROME 111 residues - 12115.1615 Da.

1

MET

THR

LEU

THR

TRP

ALA

THR

LEU

SER

2

ASP

LYS

ARG

LEU

CYS

ALA

ASP

PRO

LYS

CYS

3

GLU

GLN

ILE

SER

MET

GLY

ILE

ALA

LYS

4

ILE

THR

TYR

ALA

ILE

GLY

GLU

GLY

LEU

5

ILE

SER

PHE

LYS

ILE

ASN

SER

PRO

ILE

ARG

6

VAL

LEU

SER

LYS

SER

ALA

GLY

SER

ASN

MET

7

GLN

LEU

TRP

GLY

VAL

ASP

ILE

ASN

GLY

ARG

8

ARG

GLY

TYR

ALA

ASN

LYS

ASP

PHE

ILE

MET

9

GLU

LYS

ILE

LEU

VAL

ARG

ASP

LYS

ASP

10

LEU

TYR

GLU

VAL

PRO

VAL

GLY

PRO

11

GLY

SER

PRO

VAL

GLN

SER

VAL

GLU

THR

PRO

12

VAL

Samples:

sample_1: TANGO_DROME, [U-100% 13C; U-100% 15N], 1.0000 mM; sodium azide 0.0002 kg/m3; NaCl 137.0000 mM; KCl 2.7000 mM; Disodiumhydrogenphosphate 10.0000 mM; Potassium dihydrogen phosphate 1.8000 mM

sample_conditions_1: ionic strength: 166.500 mM; pH: 7.400; pressure: 1.000 atm; temperature: 298.000 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC/HMQC	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
HNcoCACB (H[N[co[{CA\|ca[C]}]]])	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
HNcaCO (H[N[ca[CO]]])	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
hCCcoNH (hC_cacoNH.relayed)	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1

Software:

CcpNmr Analysis v2.4 - chemical shift assignment

NMR spectrometers:

Bruker Avance 700 MHz
Bruker DRX 600 MHz
Bruker AVANCE NEO 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks