BMRB Entry 5187
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5187
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Tanaka, Takeshi; Sugai, Mariko; Kobayashi, Kuniko; Kataoka, Masakazu; Kohno, Toshiyuki. "Letter to the Editor: Complete 1H, 13C, and 15N assignments of the N-terminal
DNA binding domain of the TraR protein" J. Biomol. NMR 23, 161-162 (2002).
PubMed: 12153045
Assembly members:
Streptmycal repressor, polymer, 102 residues, 10710 Da.
Natural source: Common Name: Streptomyces nigrifaciens Taxonomy ID: 44289 Superkingdom: Eubacteria Kingdom: not available Genus/species: Streptomyces nigrifaciens
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Streptmycal repressor: GPMPYKAPEGKGYADVATHF
RTLIKSGELAPGDTLPSVAD
IRAQFGVAAKTVSRALAVLK
SEGLVSSRGALGTVVEKNPI
VITGADRLKRMEKNGMRYAP
GE
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 389 |
| 15N chemical shifts | 92 |
| 1H chemical shifts | 581 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | TraR | 1 |
Entities:
Entity 1, TraR 102 residues - 10710 Da.
| 1 | GLY | PRO | MET | PRO | TYR | LYS | ALA | PRO | GLU | GLY | ||||
| 2 | LYS | GLY | TYR | ALA | ASP | VAL | ALA | THR | HIS | PHE | ||||
| 3 | ARG | THR | LEU | ILE | LYS | SER | GLY | GLU | LEU | ALA | ||||
| 4 | PRO | GLY | ASP | THR | LEU | PRO | SER | VAL | ALA | ASP | ||||
| 5 | ILE | ARG | ALA | GLN | PHE | GLY | VAL | ALA | ALA | LYS | ||||
| 6 | THR | VAL | SER | ARG | ALA | LEU | ALA | VAL | LEU | LYS | ||||
| 7 | SER | GLU | GLY | LEU | VAL | SER | SER | ARG | GLY | ALA | ||||
| 8 | LEU | GLY | THR | VAL | VAL | GLU | LYS | ASN | PRO | ILE | ||||
| 9 | VAL | ILE | THR | GLY | ALA | ASP | ARG | LEU | LYS | ARG | ||||
| 10 | MET | GLU | LYS | ASN | GLY | MET | ARG | TYR | ALA | PRO | ||||
| 11 | GLY | GLU |
Samples:
sample_1: Streptmycal repressor, [U-99% 13C; U-99% 15N], 1.0 mM
Ex-cond_1: pH: 6.5 na; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-15N HSQC | not available | not available | not available |
| CBCA(CO)NH | not available | not available | not available |
| HNCACB | not available | not available | not available |
| HN(CO)CA | not available | not available | not available |
| HNCA | not available | not available | not available |
| HN(CA)CO | not available | not available | not available |
| HNCO | not available | not available | not available |
| C(CO)NH | not available | not available | not available |
| HBHA(CBCACO)NH | not available | not available | not available |
| HBHANH | not available | not available | not available |
| H(CCO)NH | not available | not available | not available |
| HCCH-TOCSY | not available | not available | not available |
Software:
AZARA v2.5 -
ANSIG v3.3 -
NMR spectrometers:
- Bruker AVANCE 500 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks