BMRB Entry 5185

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5185
MolProbity Validation Chart

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Title:
1H, 13C, and 15N resonance assignment of the vascular endothelial growth factor receptor-binding domain in complex with a receptor-blocking peptide
Deposition date:
2001-10-19
Original release date:
2002-05-07
Authors:
Pan, Borlan; Fairbrother, Wayne
Citation:

Citation: Pan, Borlan; Fairbrother, Wayne. "Letter to the Editor: 1H, 13C, and 15N Resonance Assignment of the Vascular Endothelial Growth Factor Receptor-binding Domain in Complex with a Receptor-blocking Peptide"  J. Biomol. NMR 22, 189-190 (2002).
PubMed: 11883783

Assembly members:

Assembly members:
vascular endothelial growth factor, polymer, 99 residues, 11625.4 Da.
v107 peptide, polymer, 19 residues, 2343.0 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
vascular endothelial growth factor: HHEVVKFMDVYQRSYCHPIE TLVDIFQEYPDEIEYIFKPS CVPLMRCGGCCNDEGLECVP TEESNITMQIMRIKPHQGQH IGEMSFLQHNKCECRPKKD
v107 peptide: GGNECDIARMWEWECFERL

Data sets:
Data typeCount
1H chemical shifts693
13C chemical shifts444
15N chemical shifts97

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1VEGF subunit 11
2VEGF subunit 21
3v107 subunit 12
4v107 subunit 22

Entities:

Entity 1, VEGF subunit 1 99 residues - 11625.4 Da.

1   HISHISGLUVALVALLYSPHEMETASPVAL
2   TYRGLNARGSERTYRCYSHISPROILEGLU
3   THRLEUVALASPILEPHEGLNGLUTYRPRO
4   ASPGLUILEGLUTYRILEPHELYSPROSER
5   CYSVALPROLEUMETARGCYSGLYGLYCYS
6   CYSASNASPGLUGLYLEUGLUCYSVALPRO
7   THRGLUGLUSERASNILETHRMETGLNILE
8   METARGILELYSPROHISGLNGLYGLNHIS
9   ILEGLYGLUMETSERPHELEUGLNHISASN
10   LYSCYSGLUCYSARGPROLYSLYSASP

Entity 2, v107 subunit 1 19 residues - 2343.0 Da.

1   GLYGLYASNGLUCYSASPILEALAARGMET
2   TRPGLUTRPGLUCYSPHEGLUARGLEU

Samples:

Sample_1: vascular endothelial growth factor, [U-98% 13C; U-98% 15N], 2.0 mM; v107 peptide 2.25 mM; H2O 90%; D2O 10%

Sample_2: vascular endothelial growth factor, [U-98% 13C; U-98% 15N], 2.0 mM; v107 peptide 2.25 mM; D2O 100%

Cond_1: pH: 7.0; temperature: 318 K

Experiments:

NameSampleSample stateSample conditions

Software:

No software information available

NMR spectrometers:

  • Bruker DRX 600 MHz
  • Bruker DRX 800 MHz

Related Database Links:

PDB
BMRB 5198

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