BMRB Entry 51835

Name: Binding of Rho GDP-dissociation inhibitor to its GTPase clients relies on preformed secondary structural properties of the binding interface
Published: 2024-06-27

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51835

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Binding of Rho GDP-dissociation inhibitor to its GTPase clients relies on preformed secondary structural properties of the binding interface

Deposition date:

2023-02-16

Original release date:

2024-06-27

Authors:

Medina-Gomez, Sara; Visco, Ilaria; Merino, Felipe; Bieling, Peter; Linser, Rasmus

Citation:

Citation: Medina-Gomez, Sara; Visco, Ilaria; Merino, Felipe; Bieling, Peter; Linser, Rasmus. "Transient Structural Properties of the Rho GDP-Dissociation Inhibitor" Angew. Chem. Int. Ed. Engl. 63, e202403941-e202403941 (2024).
PubMed: 38853146

Assembly members:

Assembly members:
entity_1, polymer, 208 residues, Formula weight is not available

Natural source:

Natural source: Common Name: cow Taxonomy ID: 9913 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Bos taurus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX6P

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GPLGMAEQEPTAEQLAQIAA ENEEDEHSVNYKPPAQKSIQ EIQELDKDDESLRKYKEALL GRVAVSADPNVPNVVVTRLT LVSSTAPGPLELDLTGDLES FKKQSFVLKEGVEYRIKISF RVNREIVSGMKYIQHTYRKG VKIDKTDYMVGSYGPRAEEY EFLTPMEEAPKGMLARGSYN IKSRFTDDDRTDHLSWEWNL TIKKEWKD

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	505
15N chemical shifts	156
1H chemical shifts	156

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	RhoGDI	1

Entities:

Entity 1, RhoGDI 208 residues - Formula weight is not available

1

GLY

PRO

LEU

GLY

MET

ALA

GLU

GLN

GLU

PRO

2

THR

ALA

GLU

GLN

LEU

ALA

GLN

ILE

ALA

3

GLU

ASN

GLU

ASP

GLU

HIS

SER

VAL

ASN

4

TYR

LYS

PRO

ALA

GLN

LYS

SER

ILE

GLN

5

GLU

ILE

GLN

GLU

LEU

ASP

LYS

ASP

GLU

6

SER

LEU

ARG

LYS

TYR

LYS

GLU

ALA

LEU

7

GLY

ARG

VAL

ALA

VAL

SER

ALA

ASP

PRO

ASN

8

VAL

PRO

ASN

VAL

THR

ARG

LEU

THR

9

LEU

VAL

SER

THR

ALA

PRO

GLY

PRO

LEU

10

GLU

LEU

ASP

LEU

THR

GLY

ASP

LEU

GLU

SER

11

PHE

LYS

GLN

SER

PHE

VAL

LEU

LYS

GLU

12

GLY

VAL

GLU

TYR

ARG

ILE

LYS

ILE

SER

PHE

13

ARG

VAL

ASN

ARG

GLU

ILE

VAL

SER

GLY

MET

14

LYS

TYR

ILE

GLN

HIS

THR

TYR

ARG

LYS

GLY

15

VAL

LYS

ILE

ASP

LYS

THR

ASP

TYR

MET

VAL

16

GLY

SER

TYR

GLY

PRO

ARG

ALA

GLU

TYR

17

GLU

PHE

LEU

THR

PRO

MET

GLU

ALA

PRO

18

LYS

GLY

MET

LEU

ALA

ARG

GLY

SER

TYR

ASN

19

ILE

LYS

SER

ARG

PHE

THR

ASP

ARG

20

THR

ASP

HIS

LEU

SER

TRP

GLU

TRP

ASN

LEU

21

THR

ILE

LYS

GLU

TRP

LYS

ASP

Samples:

sample_1: RhoGDI1, [U-13C; U-15N; U-2H], 600 uM; HEPES 20 mM; potassium chloride 150 mM; TCEP 0.5 mM; D2O, [U-100% 2H], 10%

sample_conditions_1: ionic strength: 0.15 M; pH: 7.4; pressure: 1 atm; temperature: 287 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1

Software:

CcpNMR v3.1.0 - chemical shift assignment

NMR spectrometers:

Bruker AVANCE NEO 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks