BMRB Entry 51833

Name: Differential Structural Features of Two Mutant ADAR1p150 Za Domains Cause Aicardi-Goutieres Syndrome
Published: 2023-03-14

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PDB ID: 8gbc
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51833
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Differential Structural Features of Two Mutant ADAR1p150 Za Domains Cause Aicardi-Goutieres Syndrome

Deposition date:

2023-02-16

Original release date:

2023-03-14

Authors:

Langeberg, Conner; Nichols, Parker; Henen, Morkos; Vicens, Quentin; Vogeli, Beat

Citation:

Citation: Langeberg, Conner; Nichols, Parker; Henen, Morkos; Vicens, Quentin; Vogeli, Beat. "Differential Structural Features of Two Mutant ADAR1p150 Za Domains Cause Aicardi-Goutieres Syndrome" J. Mol. Biol. 435, 168040-168040 (2023).
PubMed: 36889460

Assembly members:

Assembly members:
entity_1, polymer, 65 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28a(+)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: HMEQRILKFLEELGEGKATT AHDLSGKLGTPKKEISRVLY SLAKKGKLQKEAGTPPLWKI AVSTQ

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	154
15N chemical shifts	65
1H chemical shifts	300

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	hADARp150 Zalpha N173S	1

Entities:

Entity 1, hADARp150 Zalpha N173S 65 residues - Formula weight is not available

1

HIS

MET

GLU

GLN

ARG

ILE

LEU

LYS

PHE

LEU

2

GLU

LEU

GLY

GLU

GLY

LYS

ALA

THR

3

ALA

HIS

ASP

LEU

SER

GLY

LYS

LEU

GLY

THR

4

PRO

LYS

GLU

ILE

SER

ARG

VAL

LEU

TYR

5

SER

LEU

ALA

LYS

GLY

LYS

LEU

GLN

LYS

6

GLU

ALA

GLY

THR

PRO

LEU

TRP

LYS

ILE

7

ALA

VAL

SER

THR

GLN

Samples:

sample_1: hADAR1p150 Zalpha N173S, [U-100% 13C; U-100% 15N], 2 mM; Phosphate buffer 25 mM; NaCl 75 mM

sample_conditions_1: ionic strength: 100 mM; pH: 6.4; pressure: 1 atm; temperature: 277 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNHAHB	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1

Software:

CcpNMR - chemical shift assignment

CYANA - structure solution

NMR spectrometers:

Varian INOVA 900 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks