BMRB Entry 51828

Name: Backbone assignment of Mus Musculus FAM122A 1-124
Published: 2023-10-19

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51828

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone assignment of Mus Musculus FAM122A 1-124

Deposition date:

2023-02-10

Original release date:

2023-10-19

Authors:

Vos, Margaret; Page, Rebecca; Peti, Wolfgang

Citation:

Citation: Padi, Sathish; Vos, Margaret; Godek, Rachel; Fuller, James; Kruse, Thomas; Hein, Jamin; Nilsson, Jakob; Kelker, Matthew; Page, Rebecca; Peti, Wolfgang. "Cryo-EM structures of PP2A:B55-FAM122A and PP2A:B55-ARPP19" Nature 625, 195-203 (2024).
PubMed: 38123684

Assembly members:

Assembly members:
entity_1, polymer, 126 residues, 13635 Da.

Natural source:

Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pTHMT

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GHMAQEKMELDLELPAGASP AEGGGPGGGGLRRSNSAPLI HGLSDSSPVFQAEAPSARRN STTFPSRHGLLLPASPVRMH SSRLHQIKQEEGMDLINRET VHEREVQTAMQISHSWEESF SLSDND

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	325
15N chemical shifts	113
1H chemical shifts	113

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	FAM122A 1-124	1

Entities:

Entity 1, FAM122A 1-124 126 residues - 13635 Da.

FAM122A residues 1-124 starting with MAQEK from Mus musculus.

1

GLY

HIS

MET

ALA

GLN

GLU

LYS

MET

GLU

LEU

2

ASP

LEU

GLU

LEU

PRO

ALA

GLY

ALA

SER

PRO

3

ALA

GLU

GLY

PRO

GLY

4

LEU

ARG

SER

ASN

SER

ALA

PRO

LEU

ILE

5

HIS

GLY

LEU

SER

ASP

SER

PRO

VAL

PHE

6

GLN

ALA

GLU

ALA

PRO

SER

ALA

ARG

ASN

7

SER

THR

PHE

PRO

SER

ARG

HIS

GLY

LEU

8

LEU

PRO

ALA

SER

PRO

VAL

ARG

MET

HIS

9

SER

ARG

LEU

HIS

GLN

ILE

LYS

GLN

GLU

10

GLU

GLY

MET

ASP

LEU

ILE

ASN

ARG

GLU

THR

11

VAL

HIS

GLU

ARG

GLU

VAL

GLN

THR

ALA

MET

12

GLN

ILE

SER

HIS

SER

TRP

GLU

SER

PHE

13

SER

LEU

SER

ASP

ASN

ASP

Samples:

sample_1: FAM122A 1-124, [U-99% 15N], 150 uM; FAM122A 1-124, [U-99% 13C; U-99% 15N], 150 uM; Na2HPO4 20 mM; NaCl 150 mM; TCEP 0.5 mM

sample_conditions_1: ionic strength: 150 mM; pH: 6.3; pressure: 1 atm; temperature: 283 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN v4.1 - collection, processing

CARA v1.8.4.2 - chemical shift assignment, peak picking

NMR spectrometers:

Bruker AVANCE NEO 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks