BMRB Entry 51823

Name: Backbone assignment of MAGEA4-MHD
Published: 2024-02-06

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51823

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone assignment of MAGEA4-MHD

Deposition date:

2023-02-05

Original release date:

2024-02-06

Authors:

Alvarez, Lucia; Hennig, Janosch; Simon, Bernd; Schweimer, Kristian; Griffith-Jones, Simonne; Bhogaraju, Sagar

Citation:

Citation: Griffith-Jones, Simonne; Alvarez, Lucia; Mukhopadhyay, Urbi; Gharbi, Sarah; Rettel, Mandy; Adams, Michael; Hennig, Janosch; Bhogaraju, Sagar. "Structural basis for RAD18 regulation by MAGEA4 and its implications for RING ubiquitin ligase binding by MAGE family proteins" EMBO J. 43, 1273-1300 (2024).
PubMed: 38448672

Assembly members:

Assembly members:
entity_1, polymer, 236 residues, 26877.74 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pCoofy1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MKHHHHHHSAGLEVLFQGPD AESLFREALSNKVDELAHFL LRKYRAKELVTKAEMLERVI KNYKRCFPVIFGKASESLKM IFGIDVKEVDPTSNTYTLVT CLGLSYDGLLGNNQIFPKTG LLIIVLGTIAMEGDSASEEE IWEELGVMGVYDGREHTVYG EPRKLLTQDWVQENYLEYRQ VPGSNPARYEFLWGPRALAE TSYVKVLEHVVRVNARVRIA YPSLREAALLEEEEGV

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
15N chemical shifts	170
1H chemical shifts	170

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	MAGEA4-MHD	1

Entities:

Entity 1, MAGEA4-MHD 236 residues - 26877.74 Da.

1

MET

LYS

HIS

SER

ALA

2

GLY

LEU

GLU

VAL

LEU

PHE

GLN

GLY

PRO

ASP

3

ALA

GLU

SER

LEU

PHE

ARG

GLU

ALA

LEU

SER

4

ASN

LYS

VAL

ASP

GLU

LEU

ALA

HIS

PHE

LEU

5

LEU

ARG

LYS

TYR

ARG

ALA

LYS

GLU

LEU

VAL

6

THR

LYS

ALA

GLU

MET

LEU

GLU

ARG

VAL

ILE

7

LYS

ASN

TYR

LYS

ARG

CYS

PHE

PRO

VAL

ILE

8

PHE

GLY

LYS

ALA

SER

GLU

SER

LEU

LYS

MET

9

ILE

PHE

GLY

ILE

ASP

VAL

LYS

GLU

VAL

ASP

10

PRO

THR

SER

ASN

THR

TYR

THR

LEU

VAL

THR

11

CYS

LEU

GLY

LEU

SER

TYR

ASP

GLY

LEU

12

GLY

ASN

GLN

ILE

PHE

PRO

LYS

THR

GLY

13

LEU

ILE

VAL

LEU

GLY

THR

ILE

ALA

14

MET

GLU

GLY

ASP

SER

ALA

SER

GLU

15

ILE

TRP

GLU

LEU

GLY

VAL

MET

GLY

VAL

16

TYR

ASP

GLY

ARG

GLU

HIS

THR

VAL

TYR

GLY

17

GLU

PRO

ARG

LYS

LEU

THR

GLN

ASP

TRP

18

VAL

GLN

GLU

ASN

TYR

LEU

GLU

TYR

ARG

GLN

19

VAL

PRO

GLY

SER

ASN

PRO

ALA

ARG

TYR

GLU

20

PHE

LEU

TRP

GLY

PRO

ARG

ALA

LEU

ALA

GLU

21

THR

SER

TYR

VAL

LYS

VAL

LEU

GLU

HIS

VAL

22

VAL

ARG

VAL

ASN

ALA

ARG

VAL

ARG

ILE

ALA

23

TYR

PRO

SER

LEU

ARG

GLU

ALA

LEU

24

GLU

GLY

VAL

Samples:

sample_1: MAGEA4-MHD, [U-100% 13C; U-100% 15N; U-80% 2H], 174 uM; NaCl 200 mM; HEPES 20 mM; TCEP 1 mM

sample_conditions_1: ionic strength: 0.2 M; pH: 7.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N TROSY	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D HN(CO)CACB	sample_1	isotropic	sample_conditions_1

Software:

CARA v1.9.1.7 - chemical shift assignment

NMRPipe - processing

NMR spectrometers:

Bruker AVANCE III 800 MHz