BMRB Entry 5182

Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignments for the Extracellular Ligand-Binding Domain of Ionotropic Glutamate Receptor 2
Deposition date:
2001-10-17
Original release date:
2002-10-17
Authors:
McFeeters, Robert; Swapna, G.V.T.; Montelione, Gaetano; Oswald, Robert
Citation:

Citation: McFeeters, Robert; Swapna, G.V.T.; Montelione, Gaetano; Oswald, Robert. "Letter to the Editor: Semi-automated Backbone Resonance Assignments of the Extracellular Ligand-binding Domain of an Ionotropic Glutamate Receptor"  J. Biomol. NMR 22, 297-298 (2002).
PubMed: 11991359

Assembly members:

Assembly members:
ionotropic glutamate receptor 2, polymer, 263 residues, 29184 Da.

Natural source:

Natural source:   Common Name: Rat   Taxonomy ID: 10116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvegicus

Experimental source:

Experimental source:   Production method: recombinant technology

Data sets:
Data typeCount
13C chemical shifts740
15N chemical shifts246
1H chemical shifts246

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1gluR2 s1s21

Entities:

Entity 1, gluR2 s1s2 263 residues - 29184 Da.

1   GLYALAASNLYSTHRVALVALVALTHRTHR
2   ILELEUGLUSERPROTYRVALMETMETLYS
3   LYSASNHISGLUMETLEUGLUGLYASNGLU
4   ARGTYRGLUGLYTYRCYSVALASPLEUALA
5   ALAGLUILEALALYSHISCYSGLYPHELYS
6   TYRLYSLEUTHRILEVALGLYASPGLYLYS
7   TYRGLYALAARGASPALAASPTHRLYSILE
8   TRPASNGLYMETVALGLYGLULEUVALTYR
9   GLYLYSALAASPILEALAILEALAPROLEU
10   THRILETHRLEUVALARGGLUGLUVALILE
11   ASPPHESERLYSPROPHEMETSERLEUGLY
12   ILESERILEMETILELYSLYSGLYTHRPRO
13   ILEGLUSERALAGLUASPLEUSERLYSGLN
14   THRGLUILEALATYRGLYTHRLEUASPSER
15   GLYSERTHRLYSGLUPHEPHEARGARGSER
16   LYSILEALAVALPHEASPLYSMETTRPTHR
17   TYRMETARGSERALAGLUPROSERVALPHE
18   VALARGTHRTHRALAGLUGLYVALALAARG
19   VALARGLYSSERLYSGLYLYSTYRALATYR
20   LEULEUGLUSERTHRMETASNGLUTYRILE
21   GLUGLNARGLYSPROCYSASPTHRMETLYS
22   VALGLYGLYASNLEUASPSERLYSGLYTYR
23   GLYILEALATHRPROLYSGLYSERSERLEU
24   GLYASNALAVALASNLEUALAVALLEULYS
25   LEUASNGLUGLNGLYLEULEUASPLYSLEU
26   LYSASNLYSTRPTRPTYRASPLYSGLYGLU
27   CYSGLYSER

Samples:

sample_1: ionotropic glutamate receptor 2, [U-98% 13C; U-98% 15N; U-95% 2H], 0.45 – 0.65 mM

condition_1: pH: 5.0; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
HNCAnot availablenot availablenot available
HN(CO)CAnot availablenot availablenot available
HNCACBnot availablenot availablenot available
HN(CO)CACBnot availablenot availablenot available
HNCOnot availablenot availablenot available
HN(CA)COnot availablenot availablenot available
1H-15N HSQCnot availablenot availablenot available

Software:

nmrDraw - processing

nmrPipe - processing

Sparky - visualization

AutoAssign - automatic sequence assignment

NMR spectrometers:

  • Varian Inova 600 MHz

Related Database Links:

BMRB 15492 15493 15494 15495 15496
PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks