BMRB Entry 51814
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51814
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Citation: Neblik, Jonas; Kirupakaran, Abbna; Beuck, Christine; Mieres-Perez, Joel; Niemeyer, Felix; Le, My-Hue; Telgheder, Ursula; Schmuck, Jessica Felice; Dudziak, Alexander; Bayer, Peter; Sanchez-Garcia, Elsa; Westermann, Stefan; Schrader, Thomas. "Multivalent Molecular Tweezers Disrupt the Essential NDC80 Interaction with Microtubules" J. Am. Chem. Soc. 145, 15251-15264 (2023).
PubMed: 37392180
Assembly members:
entity_1, polymer, 128 residues, 15362 Da.
Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28a
Entity Sequences (FASTA):
entity_1: MGRDPRPLRDKNFQSAIQEE
IYDYLKKNKFDIETNHPISI
KFLKQPTQKGFIIIFKWLYL
RLDPGYGFTKSIENEIYQIL
KNLRYPFLESINKSQISAVG
GSNWHKFLGMLHWMVRTNIK
LDENLYFQ
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 336 |
| 15N chemical shifts | 116 |
| 1H chemical shifts | 116 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Ndc80 CH domain | 1 |
Entities:
Entity 1, Ndc80 CH domain 128 residues - 15362 Da.
Residues -2 to -1 and 234 to 239 (remains of TEV site) represent cloning artifacts.
| 1 | MET | GLY | ARG | ASP | PRO | ARG | PRO | LEU | ARG | ASP | ||||
| 2 | LYS | ASN | PHE | GLN | SER | ALA | ILE | GLN | GLU | GLU | ||||
| 3 | ILE | TYR | ASP | TYR | LEU | LYS | LYS | ASN | LYS | PHE | ||||
| 4 | ASP | ILE | GLU | THR | ASN | HIS | PRO | ILE | SER | ILE | ||||
| 5 | LYS | PHE | LEU | LYS | GLN | PRO | THR | GLN | LYS | GLY | ||||
| 6 | PHE | ILE | ILE | ILE | PHE | LYS | TRP | LEU | TYR | LEU | ||||
| 7 | ARG | LEU | ASP | PRO | GLY | TYR | GLY | PHE | THR | LYS | ||||
| 8 | SER | ILE | GLU | ASN | GLU | ILE | TYR | GLN | ILE | LEU | ||||
| 9 | LYS | ASN | LEU | ARG | TYR | PRO | PHE | LEU | GLU | SER | ||||
| 10 | ILE | ASN | LYS | SER | GLN | ILE | SER | ALA | VAL | GLY | ||||
| 11 | GLY | SER | ASN | TRP | HIS | LYS | PHE | LEU | GLY | MET | ||||
| 12 | LEU | HIS | TRP | MET | VAL | ARG | THR | ASN | ILE | LYS | ||||
| 13 | LEU | ASP | GLU | ASN | LEU | TYR | PHE | GLN |
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