BMRB Entry 51814

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51814

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Title:
Backbone Chemical Shift Assignments (1H, 13C, and 15N) for the CH Domain of Yeast Kinetochore Protein Ndc80
Deposition date:
2023-01-31
Original release date:
2023-08-31
Authors:
Beuck, Christine; Bayer, Peter; Schmuck, Jessica
Citation:

Citation: Neblik, Jonas; Kirupakaran, Abbna; Beuck, Christine; Mieres-Perez, Joel; Niemeyer, Felix; Le, My-Hue; Telgheder, Ursula; Schmuck, Jessica Felice; Dudziak, Alexander; Bayer, Peter; Sanchez-Garcia, Elsa; Westermann, Stefan; Schrader, Thomas. "Multivalent Molecular Tweezers Disrupt the Essential NDC80 Interaction with Microtubules"  J. Am. Chem. Soc. 145, 15251-15264 (2023).
PubMed: 37392180

Assembly members:

Assembly members:
entity_1, polymer, 128 residues, 15362 Da.

Natural source:

Natural source:   Common Name: baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MGRDPRPLRDKNFQSAIQEE IYDYLKKNKFDIETNHPISI KFLKQPTQKGFIIIFKWLYL RLDPGYGFTKSIENEIYQIL KNLRYPFLESINKSQISAVG GSNWHKFLGMLHWMVRTNIK LDENLYFQ

Data sets:
Data typeCount
13C chemical shifts336
15N chemical shifts116
1H chemical shifts116

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Ndc80 CH domain1

Entities:

Entity 1, Ndc80 CH domain 128 residues - 15362 Da.

Residues -2 to -1 and 234 to 239 (remains of TEV site) represent cloning artifacts.

1   METGLYARGASPPROARGPROLEUARGASP
2   LYSASNPHEGLNSERALAILEGLNGLUGLU
3   ILETYRASPTYRLEULYSLYSASNLYSPHE
4   ASPILEGLUTHRASNHISPROILESERILE
5   LYSPHELEULYSGLNPROTHRGLNLYSGLY
6   PHEILEILEILEPHELYSTRPLEUTYRLEU
7   ARGLEUASPPROGLYTYRGLYPHETHRLYS
8   SERILEGLUASNGLUILETYRGLNILELEU
9   LYSASNLEUARGTYRPROPHELEUGLUSER
10   ILEASNLYSSERGLNILESERALAVALGLY
11   GLYSERASNTRPHISLYSPHELEUGLYMET
12   LEUHISTRPMETVALARGTHRASNILELYS
13   LEUASPGLUASNLEUTYRPHEGLN

Samples:

sample_1: sodium phosphate 25 mM; sodium chloride 300 mM; H2O 90%; D2O, [U-99% 2H], 10%; DSS 0.1 mM; Ndc80 CH Domain, [U-98% 13C; U-98% 15N], 1.2 mM

sample_2: sodium phosphate 25 mM; sodium chloride 300 mM; H2O 90%; D2O, [U-99% 2H], 10%; DSS 0.1 mM; Ndc80 CH Domain, [U-98% 15N], 1.1 mM

sample_3: sodium phosphate 25 mM; sodium chloride 300 mM; H2O 90%; D2O, [U-99% 2H], 10%; DSS 0.1 mM; Ndc80 CH Domain, [U-98% 15N], 1.1 mM

sample_conditions_1: ionic strength: 0.3 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_3isotropicsample_conditions_1
3D 1H-15N TOCSYsample_3isotropicsample_conditions_1
3D 1H-15N NOESYsample_3isotropicsample_conditions_1
2D 1H-15N HSQCsample_2isotropicsample_conditions_1

Software:

TOPSPIN v3.5 - collection, processing

CARA v1.9 - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

  • Bruker Avance 700 MHz

Related Database Links:

UNP P40460

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks