BMRB Entry 51813

Name: HN assignments of C8i-acyl carrier protein of Burkholderia mallei
Published: 2023-05-01

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51813

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

HN assignments of C8i-acyl carrier protein of Burkholderia mallei

Deposition date:

2023-01-30

Original release date:

2023-05-01

Authors:

Fischer, Patrick; Arthanari, Hari

Citation:

Citation: Matosin, Srdan; Fischer, Patrick; Droemer, Maxim; Baggs, Eric; Chowdhury, Abu Sayeed; Tavares, Isidoro; Ficarro, Scott; Warner, Lisa Rose; Arthanari, Haribabu; Nagarajan, Rajesh. "1H, 13C and 15N backbone and sidechain assignment of the Burkholderia mallei acyl carrier protein" Biomol. NMR Assign. 17, 167-171 (2023).
PubMed: 37233945

Assembly members:

Assembly members:
entity_1, polymer, 79 residues, Formula weight is not available
entity_2, non-polymer, Formula weight is not available

Natural source:

Natural source: Common Name: Burkholderia mallei Taxonomy ID: 13373 Superkingdom: Bacteria Kingdom: not available Genus/species: Burkholderia mallei

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MDNIEQRVKKIVAEQLGVAE AEIKNEASFVNDLGADSLDT VELVMALEDEFGMEIPDEEA EKITTVQQAIDYARANVKA

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	2
15N chemical shifts	77
1H chemical shifts	76

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	C8i ACP	1
2	C8i CoA	2

Entities:

Entity 1, C8i ACP 79 residues - Formula weight is not available

1

MET

ASP

ASN

ILE

GLU

GLN

ARG

VAL

LYS

2

ILE

VAL

ALA

GLU

GLN

LEU

GLY

VAL

ALA

GLU

3

ALA

GLU

ILE

LYS

ASN

GLU

ALA

SER

PHE

VAL

4

ASN

ASP

LEU

GLY

ALA

ASP

SER

LEU

ASP

THR

5

VAL

GLU

LEU

VAL

MET

ALA

LEU

GLU

ASP

GLU

6

PHE

GLY

MET

GLU

ILE

PRO

ASP

GLU

ALA

7

GLU

LYS

ILE

THR

VAL

GLN

ALA

ILE

8

ASP

TYR

ALA

ARG

ALA

ASN

VAL

LYS

ALA

Entity 2, C8i CoA - C19H38N2O7PS - Formula weight is not available

1

Samples:

sample_1: C8i ACP of Burkholderia mallei, [U-99% 13C; U-99% 15N], 1 mM; C8i CoA 1 mM

sample_conditions_1: ionic strength: 25 mM; pH: 6.8; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1

Software:

CcpNMR - chemical shift assignment

NMR spectrometers:

Bruker AVANCE III 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks