BMRB Entry 51808

Title:
Stem 1 hairpin of Tytrahymena telomerase RNA
Deposition date:
2023-01-25
Original release date:
2023-06-19
Authors:
Wang, Yaqiang; Feigon, Juli
Citation:

Citation: Wang, Yaqiang; He, Yao; Wang, Yanjiao; Yang, Yuan; Singh, Mahavir; Eichhom, Catherine; Cheng, Xinyi; Jiang, Yi Xiao; Zhou, Z.Hong; Feigon, Juli. "Structure of LARP7 Protein p65-telomerase RNA Complex in Telomerase Revealed by Cryo-EM and NMR"  J. Mol. Biol. 435, 168044-168044 (2023).
PubMed: 37330293

Assembly members:

Assembly members:
entity_1, polymer, 17 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Tetrahymena Thermophilia   Taxonomy ID: 5911   Superkingdom: Eukaryota   Kingdom: not available   Genus/species: Tetrahymena Thermophilia

Experimental source:

Experimental source:   Production method: enzymatic semisynthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: AUACCCGCUUCGGCGGG

Data sets:
Data typeCount
1H chemical shifts8

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1stem 1 hairpin of Tetrahymena telomerase RNA1

Entities:

Entity 1, stem 1 hairpin of Tetrahymena telomerase RNA 17 residues - Formula weight is not available

1   AUACCCGCUU
2   CGGCGGG

Samples:

sample_1: stem 1 hairpin of Tetrahymena telomerase RNA 0.05 mM; D2O, [U-100% 2H], 10%; sodium phosphate 10 mM; potassium chloride 50 mM; H2O 90%

sample_conditions_1: ionic strength: 0.06 M; pH: 6.4; pressure: 1 atm; temperature: 283 K

Experiments:

NameSampleSample stateSample conditions
1D 1Hsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

TOPSPIN - collection

NMRFAM-SPARKY - chemical shift assignment

NMRDraw - processing

NMRPipe - processing

NMR spectrometers:

  • Bruker AVANCE III 800 MHz