BMRB Entry 51802

Name: Backbone assignment of DnaK C-terminal alpha-helical lid with C-IDR
Published: 2023-08-31

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51802

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone assignment of DnaK C-terminal alpha-helical lid with C-IDR

Deposition date:

2023-01-24

Original release date:

2023-08-31

Authors:

Liang, Qihui; Gong, Weibin; Perrett, Sarah

Citation:

Citation: Liang, Qihui; Zhang, Yiying; Zhang, Hong; Wu, Si; Gong, Weibin; Perrett, Sarah. "Reversible Redox-Dependent Conformational Switch of the C-Terminal a-Helical Lid of Human Hsp70 Observed by In-Cell NMR" ACS Chem. Biol. 18, 176-183 (2023).
PubMed: 36524733

Assembly members:

Assembly members:
entity_1, polymer, 107 residues, Formula weight is not available

Natural source:

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: N-SMT3 tagged pET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: LVQTRNQGDHLLHSTRKQVE EAGDKLPADDKTAIESALTA LETALKGEDKAAIEAKMQEL AQVSQKLMEIAQQQHAQQQT AGADASANNAKDDDVVDAEF EEVKDKK

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	316
15N chemical shifts	105
1H chemical shifts	105

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	DnaK(532-638)	1

Entities:

Entity 1, DnaK(532-638) 107 residues - Formula weight is not available

1

LEU

VAL

GLN

THR

ARG

ASN

GLN

GLY

ASP

HIS

2

LEU

HIS

SER

THR

ARG

LYS

GLN

VAL

GLU

3

GLU

ALA

GLY

ASP

LYS

LEU

PRO

ALA

ASP

4

LYS

THR

ALA

ILE

GLU

SER

ALA

LEU

THR

ALA

5

LEU

GLU

THR

ALA

LEU

LYS

GLY

GLU

ASP

LYS

6

ALA

ILE

GLU

ALA

LYS

MET

GLN

GLU

LEU

7

ALA

GLN

VAL

SER

GLN

LYS

LEU

MET

GLU

ILE

8

ALA

GLN

HIS

ALA

GLN

THR

9

ALA

GLY

ALA

ASP

ALA

SER

ALA

ASN

ALA

10

LYS

ASP

VAL

ASP

ALA

GLU

PHE

11

GLU

VAL

LYS

ASP

LYS

Samples:

sample_1: DnaK(532-638), [U-95% 13C; U-90% 15N], 0.8 mM; Na2HPO4 - NaH2PO4 25 mM; NaCl 50 mM

sample_conditions_1: ionic strength: 0.1 M; pH: 7.0; pressure: 1.0 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCACONH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCACO	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe, NMRViewJ - chemical shift assignment, processing

NMR spectrometers:

Varian DD2 600 MHz