BMRB Entry 51793

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51793

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Title:
FapC Full-Length
Deposition date:
2023-01-22
Original release date:
2023-01-27
Authors:
Byeon, Chang-Hyeock; Wang, Pang; Byeon, In-Ja; akbey, umit
Citation:

Citation: Byeon, Chang-Hyeock; Wang, Pang; Byeon, In-Ja; Akbey, Umit. "Solution-state NMR assignment and secondary structure propensity of the full length and minimalistic-truncated prefibrillar monomeric form of biofilm forming functional amyloid FapC from Pseudomonas aeruginosa"  Biomol. NMR Assign. 17, 159-165 (2023).
PubMed: 37162737

Assembly members:

Assembly members:
entity_1, polymer, 235 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Pseudomonas aeruginosa   Taxonomy ID: 287   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Pseudomonas aeruginosa

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MGPAEKWKPTPAPTGTVAAA VTDTQVSKDNKFDDTKTLNN AGANGSLSNSKGNLGANIAA GSGNQQDNAAAITSSAGDAA TVFAVADIYQESKDNKFTNK GTQNNALLNNSANNSSGNVG VNVAAGQGNQQKNNLAIVTA DGKNVAAASNTEQVSLDNHF LNEASSKHSYKPQYVVNNAG LLNSANNASGNIGVNVAAGA GNQQSNTLTLGSGCTVCAAG TGSKLAFLEHHHHHH

Data sets:
Data typeCount
13C chemical shifts417
15N chemical shifts198
1H chemical shifts764

Additional metadata:

  • Assembly
  • Samples and Experiments
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Assembly:

Entity Assembly IDEntity NameEntity ID
1FapC Full-Length1

Entities:

Entity 1, FapC Full-Length 235 residues - Formula weight is not available

Residue 1 is a nonnative Methionine. The last 8 residues are nonnative and due to the His-tag. The original aminoacids and the numbering in the FapC FapC is as: G25,P26,A27,...

1   METGLYPROALAGLULYSTRPLYSPROTHR
2   PROALAPROTHRGLYTHRVALALAALAALA
3   VALTHRASPTHRGLNVALSERLYSASPASN
4   LYSPHEASPASPTHRLYSTHRLEUASNASN
5   ALAGLYALAASNGLYSERLEUSERASNSER
6   LYSGLYASNLEUGLYALAASNILEALAALA
7   GLYSERGLYASNGLNGLNASPASNALAALA
8   ALAILETHRSERSERALAGLYASPALAALA
9   THRVALPHEALAVALALAASPILETYRGLN
10   GLUSERLYSASPASNLYSPHETHRASNLYS
11   GLYTHRGLNASNASNALALEULEUASNASN
12   SERALAASNASNSERSERGLYASNVALGLY
13   VALASNVALALAALAGLYGLNGLYASNGLN
14   GLNLYSASNASNLEUALAILEVALTHRALA
15   ASPGLYLYSASNVALALAALAALASERASN
16   THRGLUGLNVALSERLEUASPASNHISPHE
17   LEUASNGLUALASERSERLYSHISSERTYR
18   LYSPROGLNTYRVALVALASNASNALAGLY
19   LEULEUASNSERALAASNASNALASERGLY
20   ASNILEGLYVALASNVALALAALAGLYALA
21   GLYASNGLNGLNSERASNTHRLEUTHRLEU
22   GLYSERGLYCYSTHRVALCYSALAALAGLY
23   THRGLYSERLYSLEUALAPHELEUGLUHIS
24   HISHISHISHISHIS

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks