BMRB Entry 51792

Name: FapC L2R3C
Published: 2023-01-27

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51792

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

FapC L2R3C

Deposition date:

2023-01-22

Original release date:

2023-01-27

Authors:

Byeon, Chang-Hyeock; Wang, Pang; Byeon, In-Ja; Akbey, Umit

Citation:

Citation: Byeon, Chang-Hyeock; Wang, Pang; Byeon, In-Ja; Akbey, Umit. "Solution-state NMR assignment and secondary structure propensity of the full length and minimalistic-truncated prefibrillar monomeric form of biofilm forming functional amyloid FapC from Pseudomonas aeruginosa" Biomol. NMR Assign. 17, 159-165 (2023).
PubMed: 37162737

Assembly members:

Assembly members:
entity_1, polymer, 98 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Pseudomonas aeruginosa Taxonomy ID: 287 Superkingdom: Bacteria Kingdom: not available Genus/species: Pseudomonas aeruginosa

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET32

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GSGTNLAIVTADGKNVAAAS NTEQVSLDNHFLNEASSKHS YKPQYVVNNAGLLNSANNAS GNIGVNVAAGAGNQQSNTLT LGSGCTVCAAGTGSKLAF

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	250
15N chemical shifts	95
1H chemical shifts	518

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	FapC L2R3C	1

Entities:

Entity 1, FapC L2R3C 98 residues - Formula weight is not available

1

GLY

SER

GLY

THR

ASN

LEU

ALA

ILE

VAL

THR

2

ALA

ASP

GLY

LYS

ASN

VAL

ALA

SER

3

ASN

THR

GLU

GLN

VAL

SER

LEU

ASP

ASN

HIS

4

PHE

LEU

ASN

GLU

ALA

SER

LYS

HIS

SER

5

TYR

LYS

PRO

GLN

TYR

VAL

ASN

ALA

6

GLY

LEU

ASN

SER

ALA

ASN

ALA

SER

7

GLY

ASN

ILE

GLY

VAL

ASN

VAL

ALA

GLY

8

ALA

GLY

ASN

GLN

SER

ASN

THR

LEU

THR

9

LEU

GLY

SER

GLY

CYS

THR

VAL

CYS

ALA

10

GLY

THR

GLY

SER

LYS

LEU

ALA

PHE

Samples:

sample_1: FapC L2R3C, [U-13C; U-15N], 0.3 mM; DSS, [U-100% 2H], 1 mM; D2O, [U-99% 2H], 10%; DTT 30 mM; sodium phosphate 50 mM; sodium azide 0.02%

sample_conditions_1: pH: 7.5; pressure: 1 atm; temperature: 274 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH TOCSY	sample_1	isotropic	sample_conditions_1
3D C(CO)NH TOCSY	sample_1	isotropic	sample_conditions_1

Software:

CcpNMR v3.1 - chemical shift assignment

TOPSPIN v3 - collection

NMR spectrometers:

Bruker AVANCE III 600 MHz
Bruker Avance 700 MHz
Bruker Avance 900 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks