BMRB Entry 51785

Name: Solid-state NMR assignment data of TasA filaments
Published: 2023-04-01

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51785

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solid-state NMR assignment data of TasA filaments

Deposition date:

2023-01-17

Original release date:

2023-04-01

Authors:

Lindemann, Florian; Hiller, Matthias; Oschkinat, Hartmut

Citation:

Citation: Roske, Yvette; Lindemann, Florian; Diehl, Anne; Cremer, Nils; Higman, Victoria; Schlegel, Brigitte; Leidert, Martina; Driller, Kristina; Turgay, Kursad; Schmieder, Peter; Heinemann, Udo; Oschkinat, Hartmut. "TapA acts as specific chaperone in TasA filament formation by strand complementation" Proc. Natl. Acad. Sci. U.S.A. 120, e2217070120-e2217070120 (2023).
PubMed: 37068239

Assembly members:

Assembly members:
entity_1, polymer, 234 residues, 28955 Da.

Natural source:

Natural source: Common Name: Bacillus subtilis Taxonomy ID: 1423 Superkingdom: Bacteria Kingdom: not available Genus/species: Bacillus subtilis

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pCA528-His-Sumo

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: AFNDIKSKDATFASGTLDLS AKENSASVNLSNLKPGDKLT KDFQFENNGSLAIKEVLMAL NYGDFKANGGSNTSPEDFLS QFEVTLLTVGKEGGNGYPKN IILDDANLKDLYLMSAKNDA AAAEKIKKQIDPKFLNASGK VNVATIDGKTAPEYDGVPKT PTDFDQVQMEIQFKDDKTKD EKGLMVQNKYQGNSIKLQFS FEATQWNGLTIKKDHTDKDG YVKENEKAHSEDKN

Data sets:

assigned_chemical_shifts
spectral_peak_list

Data type	Count
13C chemical shifts	828
15N chemical shifts	226
1H chemical shifts	226

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Molecule 1	1

Entities:

Entity 1, Molecule 1 234 residues - 28955 Da.

1

ALA

PHE

ASN

ASP

ILE

LYS

SER

LYS

ASP

ALA

2

THR

PHE

ALA

SER

GLY

THR

LEU

ASP

LEU

SER

3

ALA

LYS

GLU

ASN

SER

ALA

SER

VAL

ASN

LEU

4

SER

ASN

LEU

LYS

PRO

GLY

ASP

LYS

LEU

THR

5

LYS

ASP

PHE

GLN

PHE

GLU

ASN

GLY

SER

6

LEU

ALA

ILE

LYS

GLU

VAL

LEU

MET

ALA

LEU

7

ASN

TYR

GLY

ASP

PHE

LYS

ALA

ASN

GLY

8

SER

ASN

THR

SER

PRO

GLU

ASP

PHE

LEU

SER

9

GLN

PHE

GLU

VAL

THR

LEU

THR

VAL

GLY

10

LYS

GLU

GLY

ASN

GLY

TYR

PRO

LYS

ASN

11

ILE

LEU

ASP

ALA

ASN

LEU

LYS

ASP

12

LEU

TYR

LEU

MET

SER

ALA

LYS

ASN

ASP

ALA

13

ALA

GLU

LYS

ILE

LYS

GLN

ILE

14

ASP

PRO

LYS

PHE

LEU

ASN

ALA

SER

GLY

LYS

15

VAL

ASN

VAL

ALA

THR

ILE

ASP

GLY

LYS

THR

16

ALA

PRO

GLU

TYR

ASP

GLY

VAL

PRO

LYS

THR

17

PRO

THR

ASP

PHE

ASP

GLN

VAL

GLN

MET

GLU

18

ILE

GLN

PHE

LYS

ASP

LYS

THR

LYS

ASP

19

GLU

LYS

GLY

LEU

MET

VAL

GLN

ASN

LYS

TYR

20

GLN

GLY

ASN

SER

ILE

LYS

LEU

GLN

PHE

SER

21

PHE

GLU

ALA

THR

GLN

TRP

ASN

GLY

LEU

THR

22

ILE

LYS

ASP

HIS

THR

ASP

LYS

ASP

GLY

23

TYR

VAL

LYS

GLU

ASN

GLU

LYS

ALA

HIS

SER

24

GLU

ASP

LYS

ASN

Samples:

sample_1: TasA filament / multimer, [U-13C; U-15N; U-2H; 100% H2O back-exchanged], solid; sodium phosphate 20 mM; sodium chloride 50 mM

sample_2: TasA filament / multimer, [U-13C; U-15N], solid; sodium phosphate 20 mM; sodium chloride 50 mM

sample_3: TasA filament / multimer, [2-13C, U-15N], solid; sodium phosphate 20 mM; sodium chloride 50 mM

sample_4: TasA filament / multimer, [1,3-13C, U-15N], solid; sodium phosphate 20 mM; sodium chloride 50 mM

sample_conditions_1: pH: 7

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC/HMQC	sample_1	solid	sample_conditions_1
3D HNCO	sample_1	solid	sample_conditions_1
3D HN(CO)CA	sample_1	solid	sample_conditions_1
Expt_9 (H[N[co[ca[CB]]]])	sample_1	solid	sample_conditions_1
Expt_13 (H[N[ca[CO]]])	sample_1	solid	sample_conditions_1
Expt_14 (H[N_[CA]].onebond)	sample_1	solid	sample_conditions_1
Expt_15 (H[N[ca[CB]]])	sample_1	solid	sample_conditions_1
C-C DARR	sample_2	solid	sample_conditions_1
C-C DARR	sample_3	solid	sample_conditions_1
C-C DARR	sample_3	solid	sample_conditions_1
C-C DARR	sample_4	solid	sample_conditions_1
C-C DARR	sample_4	solid	sample_conditions_1

Software:

CcpNmr Analysis v2.4.2 - chemical shift assignment, peak picking

TOPSPIN v3.5pl7 - collection, processing

NMR spectrometers:

Bruker AVANCE III 600 MHz
Bruker AVANCE III 800 MHz
Bruker AVANCE III 900 MHz

UniProt	P54507
AlphaFold	P54507